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Mouse Anti-NAPRT Recombinant Antibody (CBWJN-0580) (CBMAB-N1170-WJ)

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Basic Information

Host Animal
Mouse
Clone
CBWJN-0580
Application
WB, IHC, IHC-P
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Nicotinate Phosphoribosyltransferase
Introduction
Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM, Mar 2008]
Entrez Gene ID
93100
UniProt ID
Q6XQN6
Alternative Names
Nicotinate Phosphoribosyltransferase; Nicotinate Phosphoribosyltransferase Domain-Containing Protein 1; Nicotinate Phosphoribosyltransferase Domain Containing 1; FHA-HIT-Interacting Protein; NAPRTase; NAPRT1;
Function
Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate (PubMed:17604275, PubMed:21742010, PubMed:26042198).

Helps prevent cellular oxidative stress via its role in NAD biosynthesis (PubMed:17604275).
Biological Process
NAD salvage Source: GO_Central
Response to oxidative stress Source: UniProtKB
Cellular Location
Cytosol
PTM
Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.

Armandi, A., Colombo, G., Rosso, C., Caviglia, G. P., Olivero, A., Abate, M. L., ... & Bugianesi, E. (2023). The Predictive Role of Extracellular NAPRT for the Detection of Advanced Fibrosis in Biopsy-Proven Non-Alcoholic Fatty Liver Disease. International Journal of Molecular Sciences, 24(2), 1172.

Kim, M., Kim, H., Kang, B. G., Lee, J., Kim, T., Lee, H., ... & Kim, H. S. (2023). Discovery of a novel NAMPT inhibitor that selectively targets NAPRT-deficient EMT-subtype cancer cells and alleviates chemotherapy-induced peripheral neuropathy. Theranostics, 13(14), 5075.

Colombo, G., Caviglia, G. P., Ravera, A., Tribocco, E., Frara, S., Rosso, C., ... & Ribaldone, D. G. (2023). NAMPT and NAPRT serum levels predict response to anti-TNF therapy in inflammatory bowel disease. Frontiers in Medicine, 10, 1116862.

Franco, J., Piacente, F., Walter, M., Fratta, S., Ghanem, M., Benzi, A., ... & Del Rio, A. (2022). Structure-based identification and biological characterization of new NAPRT inhibitors. Pharmaceuticals, 15(7), 855.

Ghanem, M. S., Caffa, I., Del Rio, A., Franco, J., Parenti, M. D., Monacelli, F., ... & Piacente, F. (2022). Identification of NAPRT inhibitors with anti-cancer properties by in silico drug discovery. Pharmaceuticals, 15(7), 848.

Franceschini, N., Oosting, J., Tamsma, M., Niessen, B., Bruijn, I. B. D., van den Akker, B., ... & Cleton-Jansen, A. M. (2021). Targeting the NAD salvage synthesis pathway as a novel therapeutic strategy for osteosarcomas with low NAPRT expression. International Journal of Molecular Sciences, 22(12), 6273.

Audrito, V., Messana, V. G., & Deaglio, S. (2020). NAMPT and NAPRT: two metabolic enzymes with key roles in inflammation. Frontiers in Oncology, 10, 358.

Fons, N. R., Sundaram, R. K., Breuer, G. A., Peng, S., McLean, R. L., Kalathil, A. N., ... & Bindra, R. S. (2019). PPM1D mutations silence NAPRT gene expression and confer NAMPT inhibitor sensitivity in glioma. Nature communications, 10(1), 3790.

Li, X. Q., Lei, J., Mao, L. H., Wang, Q. L., Xu, F., Ran, T., ... & He, S. (2019). NAMPT and NAPRT, key enzymes in NAD salvage synthesis pathway, are of negative prognostic value in colorectal cancer. Frontiers in Oncology, 9, 736.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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