Mouse Anti-NASP Recombinant Antibody (A-7) (CBMAB-N1208-WJ)

Name: Mouse Anti-NASP Recombinant Antibody (A-7)
SKU: CBMAB-N1208-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

A-7

Application

WB, IP, IF, ELISA

Specificity

Human, Mouse, Rat

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nuclear autoantigenic sperm protein (histone-binding)

Introduction

This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	4678
Mouse	50927
Rat	298441

UniProt ID

Human	P49321
Mouse	Q99MD9
Rat	Q66HD3

Alternative Names

Nuclear Autoantigenic Sperm Protein; Nuclear Autoantigenic Sperm Protein (Histone-Binding); NASP Histone Chaperone; Histone H1-Binding Protein; FLB7527; PRO1999; HMDRA1;

Function

Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.

Biological Process

Blastocyst development Source: UniProtKB
CENP-A containing chromatin assembly Source: GO_Central
DNA replication Source: UniProtKB-KW
DNA replication-dependent chromatin assembly Source: UniProtKB
DNA replication-independent chromatin assembly Source: UniProtKB
G1/S transition of mitotic cell cycle Source: UniProtKB
Histone exchange Source: UniProtKB
Male gonad development Source: Ensembl
Nucleosome assembly Source: UniProtKB
Protein transport Source: UniProtKB-KW
Response to testosterone Source: Ensembl

Cellular Location

Nucleus
Cytoplasm

Takeuchi, H., Nagahara, S., Higashiyama, T., & Berger, F. (2023). The Chaperone NASP Contributes to De Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis. bioRxiv, 2023-10.

Zhang, Y., Yan, H., Zhu, J., Chen, L., Wang, H., & Ju, J. (2022). Hepatic fibrosis aggravation in nuclear autoantigenic sperm protein (NASP) mutant mice induced by concanavalin A. Xi bao yu fen zi Mian yi xue za zhi= Chinese Journal of Cellular and Molecular Immunology, 38(7), 577-583.

Huang, Y., Yang, S., Yu, W., & Gui, L. (2022). Somatic nuclear auto-antigenic sperm protein sensitizes human breast cancer cells to 5-Fluorouracil. Cancer Chemotherapy and Pharmacology, 89(4), 559-564.

Liu, Y., Chen, L., Wang, N., Wu, B., Bao, H., & Huang, H. (2022). Structural basis for histone H3 recognition by NASP in Arabidopsis. Journal of Integrative Plant Biology, 64(12), 2309-2313.

Zhu, Y., Li, B., Xu, G., Han, C., & Xing, G. (2021). Knockdown of long noncoding RNA colorectal neoplasia differentially expressed inhibits hepatocellular carcinoma progression by mediating the expression of nuclear autoantigenic sperm protein. Oncology reports, 46(6), 1-11.

Le Goff, S., Keçeli, B. N., Jeřábková, H., Heckmann, S., Rutten, T., Cotterell, S., ... & Lermontova, I. (2020). The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis. The Plant Journal, 101(1), 71-86.

Kong, F., Li, L., Wang, C., Zhang, Q., & He, S. (2020). MiR-381-3p suppresses biological characteristics of cancer in head-neck squamous cell carcinoma cells by targeting nuclear autoantigenic sperm protein (NASP). Bioscience, Biotechnology, and Biochemistry, 84(4), 703-713.

Li, J. X., Wei, C. Y., Cao, S. G., & Xia, M. W. (2019). Elevated nuclear auto-antigenic sperm protein promotes melanoma progression by inducing cell proliferation. OncoTargets and therapy, 12, 2105.

Zhan, Q., Zhang, J., Wang, H., Wu, X., Zhu, Y., Xu, Z., & Ju, J. (2019). Mutation of nuclear autoantigenic sperm protein (NASP) gene aggravates autoimmune response in induced lupus model mice. Xi bao yu fen zi Mian yi xue za zhi= Chinese Journal of Cellular and Molecular Immunology, 35(9), 776-782.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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