Sign in or Register   Sign in or Register
  |  

Mouse Anti-NASP Recombinant Antibody (A-7) (CBMAB-N1208-WJ)

This product is a Mouse antibody that recognizes NASP. The antibody A-7 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all NASP antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A-7
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human, Mouse, Rat
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
nuclear autoantigenic sperm protein (histone-binding)
Introduction
This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human4678
Mouse50927
Rat298441
UniProt ID
HumanP49321
MouseQ99MD9
RatQ66HD3
Alternative Names
Nuclear Autoantigenic Sperm Protein; Nuclear Autoantigenic Sperm Protein (Histone-Binding); NASP Histone Chaperone; Histone H1-Binding Protein; FLB7527; PRO1999; HMDRA1;
Function
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
Biological Process
Blastocyst development Source: UniProtKB
CENP-A containing chromatin assembly Source: GO_Central
DNA replication Source: UniProtKB-KW
DNA replication-dependent chromatin assembly Source: UniProtKB
DNA replication-independent chromatin assembly Source: UniProtKB
G1/S transition of mitotic cell cycle Source: UniProtKB
Histone exchange Source: UniProtKB
Male gonad development Source: Ensembl
Nucleosome assembly Source: UniProtKB
Protein transport Source: UniProtKB-KW
Response to testosterone Source: Ensembl
Cellular Location
Nucleus
Cytoplasm

Takeuchi, H., Nagahara, S., Higashiyama, T., & Berger, F. (2023). The Chaperone NASP Contributes to De Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis. bioRxiv, 2023-10.

Zhang, Y., Yan, H., Zhu, J., Chen, L., Wang, H., & Ju, J. (2022). Hepatic fibrosis aggravation in nuclear autoantigenic sperm protein (NASP) mutant mice induced by concanavalin A. Xi bao yu fen zi Mian yi xue za zhi= Chinese Journal of Cellular and Molecular Immunology, 38(7), 577-583.

Huang, Y., Yang, S., Yu, W., & Gui, L. (2022). Somatic nuclear auto-antigenic sperm protein sensitizes human breast cancer cells to 5-Fluorouracil. Cancer Chemotherapy and Pharmacology, 89(4), 559-564.

Liu, Y., Chen, L., Wang, N., Wu, B., Bao, H., & Huang, H. (2022). Structural basis for histone H3 recognition by NASP in Arabidopsis. Journal of Integrative Plant Biology, 64(12), 2309-2313.

Zhu, Y., Li, B., Xu, G., Han, C., & Xing, G. (2021). Knockdown of long noncoding RNA colorectal neoplasia differentially expressed inhibits hepatocellular carcinoma progression by mediating the expression of nuclear autoantigenic sperm protein. Oncology reports, 46(6), 1-11.

Le Goff, S., Keçeli, B. N., Jeřábková, H., Heckmann, S., Rutten, T., Cotterell, S., ... & Lermontova, I. (2020). The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis. The Plant Journal, 101(1), 71-86.

Kong, F., Li, L., Wang, C., Zhang, Q., & He, S. (2020). MiR-381-3p suppresses biological characteristics of cancer in head-neck squamous cell carcinoma cells by targeting nuclear autoantigenic sperm protein (NASP). Bioscience, Biotechnology, and Biochemistry, 84(4), 703-713.

Li, J. X., Wei, C. Y., Cao, S. G., & Xia, M. W. (2019). Elevated nuclear auto-antigenic sperm protein promotes melanoma progression by inducing cell proliferation. OncoTargets and therapy, 12, 2105.

Zhan, Q., Zhang, J., Wang, H., Wu, X., Zhu, Y., Xu, Z., & Ju, J. (2019). Mutation of nuclear autoantigenic sperm protein (NASP) gene aggravates autoimmune response in induced lupus model mice. Xi bao yu fen zi Mian yi xue za zhi= Chinese Journal of Cellular and Molecular Immunology, 35(9), 776-782.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-NASP Recombinant Antibody (A-7)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare