Rabbit Anti-NCF1 Recombinant Antibody (D21F6) (CBMAB-P0538-YC)

Provided herein is a Rabbit monoclonal antibody against Human Neutrophil Cytosolic Factor 1. The antibody can be used for immunoassay techniques, such as WB.
See all NCF1 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Monkey

Clone

D21F6

Antibody Isotype

IgG

Application

Basic Information

Specificity

Human, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Neutrophil Cytosolic Factor 1

Introduction

NCF1 is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.

Entrez Gene ID

Human	653361
Monkey	694207

UniProt ID

Human	P14598
Monkey	F6SL33

Alternative Names

Neutrophil Cytosolic Factor 1; 47 KDa Autosomal Chronic Granulomatous Disease Protein; SH3 And PX Domain-Containing Protein 1A; Neutrophil NADPH Oxidase Factor 1; 47 KDa Neutrophil Oxidase Factor; NADPH Oxidase Organizer 2; Nox-Organizing Protein 2; Nox Organizer 2; SH3PXD1A; P47-Phox;

Function

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Biological Process

Apoptotic process Source: Ensembl
Cellular defense response Source: ProtInc
Cellular response to cadmium ion Source: CAFA
Cellular response to reactive oxygen species Source: CAFA
Innate immune response Source: BHF-UCL
Positive regulation of epidermal growth factor-activated receptor activity Source: CAFA
Positive regulation of JNK cascade Source: CAFA
Positive regulation of p38MAPK cascade Source: CAFA
Positive regulation of phosphatidylinositol 3-kinase signaling Source: CAFA
Positive regulation of transcription, DNA-templated Source: CAFA
Protein targeting to membrane Source: UniProtKB
Reactive oxygen species biosynthetic process Source: Ensembl
Regulation of respiratory burst involved in inflammatory response Source: Ensembl
Respiratory burst Source: GO_Central
Superoxide anion generation Source: GO_Central
Superoxide metabolic process Source: BHF-UCL

Cellular Location

Cytosol
Other locations
Membrane

Involvement in disease

Granulomatous disease, chronic, autosomal recessive, 1 (CGD1):
A form of chronic granulomatous disease, a primary immunodeficiency characterized by severe recurrent bacterial and fungal infections, along with manifestations of chronic granulomatous inflammation. It results from an impaired ability of phagocytes to mount a burst of reactive oxygen species in response to pathogens.

PTM

Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.

References

Luo, H., Urbonaviciute, V., Saei, A. A., Lyu, H., Gaetani, M., Végvári, Á., ... & Holmdahl, R. (2023). NCF1-dependent production of ROS protects against lupus by regulating plasmacytoid dendritic cell development and functions. JCI insight, 8(7).

Bakry, O., Shoeib, M., Soliman, S., & Kamal, L. (2021). Neutrophil Cytosolic Factor-1 Genotyping in Acne Vulgaris. Skin Pharmacology and Physiology, 34(1), 51-56.

Linge, P., Arve, S., Olsson, L. M., Leonard, D., Sjöwall, C., Frodlund, M., ... & Bengtsson, A. (2020). NCF1-339 polymorphism is associated with altered formation of neutrophil extracellular traps, high serum interferon activity and antiphospholipid syndrome in systemic lupus erythematosus. Annals of the Rheumatic Diseases, 79(2), 254-261.

Liu, C., Whitener, R. L., Lin, A., Xu, Y., Chen, J., Savinov, A., ... & Mathews, C. E. (2019). Neutrophil cytosolic factor 1 in dendritic cells promotes autoreactive CD8+ T cell activation via cross-presentation in type 1 diabetes. Frontiers in immunology, 10, 952.

Chen, D. F., Cui, X. Z., Cao, W. M., & Meng, W. (2019). Neutrophil cytosolic factor 1 contributes to the development of sepsis. Inflammation, 42, 811-817.

Kuhns, D. B., Hsu, A. P., Sun, D., Lau, K., Fink, D., Griffith, P., ... & Gallin, J. I. (2019). NCF1 (p47phox)–deficient chronic granulomatous disease: comprehensive genetic and flow cytometric analysis. Blood advances, 3(2), 136-147.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NCF1 Recombinant Antibody (1/p47Phox)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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