Mouse Anti-NCF4 Recombinant Antibody (3E3) (CBMAB-N1389-WJ)

Name: Mouse Anti-NCF4 Recombinant Antibody (3E3)
SKU: CBMAB-N1389-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3E3

Application

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1.00 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Neutrophil Cytosolic Factor 4

Introduction

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Entrez Gene ID

4689

UniProt ID

Q15080

Alternative Names

Neutrophil Cytosolic Factor 4; Neutrophil NADPH Oxidase Factor 4; SH3 And PX Domain-Containing Protein 4; Neutrophil Cytosolic Factor 4, 40kDa; P40-Phox; P40PHOX; SH3PXD4;

Function

Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.

Biological Process

Phagocytosis Source: InterPro
Respiratory burst Source: ComplexPortal
Superoxide anion generation Source: UniProtKB

Cellular Location

Endosome
Endosome membrane
Cytosol
Other locations
Membrane

Involvement in disease

Granulomatous disease, chronic, autosomal recessive, 3 (CGD3):
A form of chronic granulomatous disease, a primary immunodeficiency characterized by severe recurrent bacterial and fungal infections, along with manifestations of chronic granulomatous inflammation. It results from an impaired ability of phagocytes to mount a burst of reactive oxygen species in response to pathogens.

Bakhshaei, F., Sharifiyazdi, H., Rowshan-Ghasrodashti, A., Zare, H. R., Mirzaei, A., & Nazifi, S. (2023). Polymorphism in neutrophil cytosolic factor 4 (NCF4) of dairy cows had mastitis in previous lactations, and the relationship with the respiratory burst. Research in Veterinary Science, 160, 39-44.

He, C., Luo, H., Coelho, A., Liu, M., Li, Q., Xu, J., ... & Holmdahl, R. (2022). NCF4 dependent intracellular reactive oxygen species regulate plasma cell formation. Redox Biology, 56, 102422.

Zhang, T. P., Li, R., Huang, Q., Pan, H. F., Ye, D. Q., & Li, X. M. (2020). Association of NCF2, NCF4, and CYBA gene polymorphisms with rheumatoid arthritis in a Chinese population. Journal of Immunology Research, 2020.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

NCF4 Matched Antibody Pair (777) (CAT#: APMAB-777LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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