Rabbit Anti-NCOA1 Recombinant Antibody (CBLY1-169) (CBMAB-K2175-LY)

Name: Rabbit Anti-NCOA1 Recombinant Antibody (CBLY1-169)
SKU: CBMAB-K2175-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBLY1-169

Application

WB, IHC-P, IP

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

NCOA1

Introduction

The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Entrez Gene ID

8648

UniProt ID

Q15788

Alternative Names

Nuclear Receptor Coactivator 1; Class E Basic Helix-Loop-Helix Protein 74; Renal Carcinoma Antigen NY-REN-52; EC 2.3.1.48; BHLHe74; RIP160; SRC1; Steroid Receptor Coactivator-1; Steroid Receptor Coactivator 1;

Function

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

Biological Process

Cellular response to hormone stimulus Source: GO_Central
Cellular response to Thyroglobulin triiodothyronine Source: Ensembl
Histone H4 acetylation Source: Ensembl
Labyrinthine layer morphogenesis Source: Ensembl
mRNA transcription by RNA polymerase II Source: ComplexPortal
Peroxisome proliferator activated receptor signaling pathway Source: ComplexPortal
Positive regulation of adipose tissue development Source: ComplexPortal
Positive regulation of apoptotic process Source: Ensembl
Positive regulation of neuron differentiation Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of transcription from RNA polymerase II promoter by galactose Source: UniProtKB
Regulation of cellular response to insulin stimulus Source: ComplexPortal
Regulation of thyroid hormone mediated signaling pathway Source: Ensembl

Involvement in disease

A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.

PTM

Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.

Li, X., Wang, X., Chen, C., Zhang, E., Zhang, Y., Li, H., ... & Zhang, F. (2023). Accumulation of NCOA1 dependent on HERC3 deficiency transactivates matrix metallopeptidases and promotes extracellular matrix degradation in intervertebral disc degeneration. Life Sciences, 320, 121555.

Mejbel, H. A., Harada, S., Stevens, T. M., Huang, X., Netto, G. J., Mackinnon, A. C., & Al Diffalha, S. (2023). Spindle cell sarcoma of the uterus harboring MEIS1:: NCOA1 fusion gene and mimicking endometrial stromal sarcoma. International Journal of Surgical Pathology, 31(2), 227-232.

Dermawan, J. K., Azzato, E. M., Jebastin Thangaiah, J., Gjorgova‐Gjeorgievski, S., Rubin, B. P., Folpe, A. L., ... & Fritchie, K. J. (2021). PRRX1–NCOA1‐rearranged fibroblastic tumour: a clinicopathological, immunohistochemical and molecular genetic study of six cases of a potentially under‐recognised, distinctive mesenchymal tumour. Histopathology, 79(6), 997-1003.

Tauziède-Espariat, A., Siegfried, A., Nicaise, Y., Kergrohen, T., Sievers, P., Vasiljevic, A., ... & RENOCLIP-LOC, the BIOMECA (Biomarkers for Ependymomas in Children, Adolescents) consortium. (2021). Supratentorial non-RELA, ZFTA-fused ependymomas: a comprehensive phenotype genotype correlation highlighting the number of zinc fingers in ZFTA-NCOA1/2 fusions. Acta Neuropathologica Communications, 9, 1-13.

Tomomasa, R., Arai, Y., Kawabata‐Iwakawa, R., Fukuoka, K., Nakano, Y., Hama, N., ... & Nobusawa, S. (2021). Ependymoma‐like tumor with mesenchymal differentiation harboring C11orf95‐NCOA1/2 or‐RELA fusion: A hitherto unclassified tumor related to ependymoma. Brain Pathology, 31(3), e12943.

Karanian, M., Pissaloux, D., Gomez-Brouchet, A., Chevenet, C., Le Loarer, F., Fernandez, C., ... & Tirode, F. (2020). SRF-FOXO1 and SRF-NCOA1 fusion genes delineate a distinctive subset of well-differentiated rhabdomyosarcoma. The American Journal of Surgical Pathology, 44(5), 607-616.

Goebel, E. A., Bonilla, S. H., Dong, F., Dickson, B. C., Hoang, L. N., Hardisson, D., ... & Kolin, D. L. (2020). Uterine tumor resembling ovarian sex cord tumor (UTROSCT): a morphologic and molecular study of 26 cases confirms recurrent NCOA1–3 rearrangement. The American journal of surgical pathology, 44(1), 30.

Grither, W. R., Dickson, B. C., Fuh, K. C., & Hagemann, I. S. (2020). Detection of a somatic GREB1-NCOA1 gene fusion in a uterine tumor resembling ovarian sex cord tumor (UTROSCT). Gynecologic Oncology Reports, 34, 100636.

Lacambra, M. D., Weinreb, I., Demicco, E. G., Chow, C., Sung, Y. S., Swanson, D., ... & Dickson, B. C. (2019). PRRX‐NCOA1/2 rearrangement characterizes a distinctive fibroblastic neoplasm. Genes, Chromosomes and Cancer, 58(10), 705-712.

Abu-Taweel, G. M., Rajagopal, R., Sun-Ju, K., Kim, H. J., Kim, Y. O., Mothana, R. A., ... & Al-Rehaily, A. J. (2019). Betulinic acid lowers lipid accumulation in adipocytes through enhanced NCoA1–PPARγ interaction. Journal of Infection and Public Health, 12(5), 726-732.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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