Mouse Anti-NDC80 Recombinant Antibody (CBT4202) (V2LY-0625-LY1822)

Name: Mouse Anti-NDC80 Recombinant Antibody (CBT4202)
SKU: V2LY-0625-LY1822
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-NDC80 Recombinant Antibody (CBT4202) (V2LY-0625-LY1822) in ELISA

Mouse Anti-NDC80 Recombinant Antibody (CBT4202) (V2LY-0625-LY1822) in WB

Mouse Anti-NDC80 Recombinant Antibody (CBT4202) (V2LY-0625-LY1822) in IF

Mouse Anti-NDC80 Recombinant Antibody (CBT4202) (V2LY-0625-LY1822) in FC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4202

Application

WB, ICC, FC

Immunogen

Purified recombinant fragment of human NDC80 (AA: 443-642) expressed in mammalian.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:200-1:1,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

NDC80 kinetochore complex component

Entrez Gene ID

10403

UniProt ID

O14777

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327, PubMed:30409912).

Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592, PubMed:30409912).

The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).

Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).

Biological Process

Attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
Attachment of spindle microtubules to kinetochore Source: ComplexPortal
Cell division Source: UniProtKB-KW
Centrosome duplication Source: MGI
Chromosome segregation Source: UniProtKB
Establishment of mitotic spindle orientation Source: UniProtKB
G2/MI transition of meiotic cell cycle Source: Ensembl
Kinetochore organization Source: CAFA
Metaphase plate congression Source: MGI
Mitotic cell cycle Source: ProtInc
Mitotic sister chromatid segregation Source: ProtInc
Mitotic spindle assembly checkpoint signaling Source: ComplexPortal
Mitotic spindle organization Source: UniProtKB
Positive regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Positive regulation of protein localization to kinetochore Source: CAFA
Regulation of protein stability Source: Ensembl
Spindle assembly involved in female meiosis I Source: Ensembl

Cellular Location

Nucleus
Other locations
kinetochore
Note: Localizes to kinetochores from late prophase to anaphase (PubMed:14699129). Localizes specifically to the outer plate of the kinetochore (PubMed:14699129).

PTM

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB.
Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment (PubMed:30409912). Deacetylated by SIRT1 (PubMed:30409912).

Zahm, J. A., Jenni, S., & Harrison, S. C. (2023). Structure of the Ndc80 complex and its interactions at the yeast kinetochore–microtubule interface. Open Biology, 13(3), 220378.

Muir, K. W., Batters, C., Dendooven, T., Yang, J., Zhang, Z., Burt, A., & Barford, D. (2023). Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules. Science, 382(6675), 1184-1190.

Polley, S., Müschenborn, H., Terbeck, M., De Antoni, A., Vetter, I. R., Dogterom, M., ... & Huis in't Veld, P. J. (2023). Stable kinetochore‐microtubule attachment requires loop‐dependent Ndc80‐Ndc80 binding. The EMBO Journal, e112504.

Sarangapani, K. K., Koch, L. B., Nelson, C. R., Asbury, C. L., & Biggins, S. (2021). Kinetochore-bound Mps1 regulates kinetochore–microtubule attachments via Ndc80 phosphorylation. Journal of Cell Biology, 220(12), e202106130.

Dong, Q., Liu, X. L., Wang, X. H., Zhao, Y., Chen, Y. H., & Li, F. (2021). Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly. Proceedings of the National Academy of Sciences, 118(48), e2104459118.

Ustinov, N. B., Korshunova, A. V., & Gudimchuk, N. B. (2020). Protein complex NDC80: Properties, functions, and possible role in pathophysiology of cell division. Biochemistry (Moscow), 85, 448-462.

Wimbish, R. T., & DeLuca, J. G. (2020). Hec1/Ndc80 tail domain function at the kinetochore-microtubule interface. Frontiers in cell and developmental biology, 8, 43.

Wimbish, R. T., DeLuca, K. F., Mick, J. E., Himes, J., Jiménez-Sánchez, I., Jeyaprakash, A. A., & DeLuca, J. G. (2020). The Hec1/Ndc80 tail domain is required for force generation at kinetochores, but is dispensable for kinetochore–microtubule attachment formation and Ska complex recruitment. Molecular Biology of the Cell, 31(14), 1453-1473.

Brusini, L., D’Archivio, S., McDonald, J., & Wickstead, B. (2019). Ndc80/Nuf2-like protein KKIP1 connects a stable kinetoplastid outer kinetochore complex to the inner kinetochore and responds to metaphase tension. BioRxiv, 764829.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

NDC80 Matched Antibody Pair (779) (CAT#: APMAB-779LY)

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Isotype control

For research use only. Not intended for any clinical use.

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