NDC80

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

NDC80 kinetochore complex component

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327, PubMed:30409912).

Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592, PubMed:30409912).

The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).

Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).

Attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
Attachment of spindle microtubules to kinetochore Source: ComplexPortal
Cell division Source: UniProtKB-KW
Centrosome duplication Source: MGI
Chromosome segregation Source: UniProtKB
Establishment of mitotic spindle orientation Source: UniProtKB
G2/MI transition of meiotic cell cycle Source: Ensembl
Kinetochore organization Source: CAFA
Metaphase plate congression Source: MGI
Mitotic cell cycle Source: ProtInc
Mitotic sister chromatid segregation Source: ProtInc
Mitotic spindle assembly checkpoint signaling Source: ComplexPortal
Mitotic spindle organization Source: UniProtKB
Positive regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Positive regulation of protein localization to kinetochore Source: CAFA
Regulation of protein stability Source: Ensembl
Spindle assembly involved in female meiosis I Source: Ensembl

Nucleus
Other locations
kinetochore
Note: Localizes to kinetochores from late prophase to anaphase (PubMed:14699129). Localizes specifically to the outer plate of the kinetochore (PubMed:14699129).

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB.
Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment (PubMed:30409912). Deacetylated by SIRT1 (PubMed:30409912).