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Mouse Anti-NDC80 (AA 56-642) Recombinant Antibody (CBFYH-3621) (CBMAB-H4179-FY)

This product is mouse antibody that recognizes NDC80. The antibody CBFYH-3621 can be used for immunoassay techniques such as: FC, IF, IHC, IP, WB.
See all NDC80 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Pig, Rat, Hamster
Clone
CBFYH-3621
Antibody Isotype
IgG2a
Application
FC, IF, IHC, IP, WB

Basic Information

Immunogen
Human HEC1 protein consisting of amino acids 56-642
Specificity
Human, Mouse, Pig, Rat, Hamster
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Concentration
0.27 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 56-642

Target

Full Name
NDC80 kinetochore complex component
Introduction
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
Entrez Gene ID
Human10403
Mouse67052
Rat301701
Pig100626545
Hamster100751668
UniProt ID
HumanO14777
MouseQ9D0F1
RatF7F189
PigF1SBC5
HamsterA0A1U8CEK1
Alternative Names
NDC80, Kinetochore Complex Component; Retinoblastoma-Associated Protein HEC; Highly Expressed In Cancer Protein; Kinetochore-Associated Protein 2; Kinetochore Associated 2; Kinetochore Protein Hec1; HsHec1; KNTC2; HEC1; HEC
Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327, PubMed:30409912).

Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592, PubMed:30409912).

The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).

Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).
Biological Process
Attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
Attachment of spindle microtubules to kinetochore Source: ComplexPortal
Cell division Source: UniProtKB-KW
Centrosome duplication Source: MGI
Chromosome segregation Source: UniProtKB
Establishment of mitotic spindle orientation Source: UniProtKB
G2/MI transition of meiotic cell cycle Source: Ensembl
Kinetochore organization Source: CAFA
Metaphase plate congression Source: MGI
Mitotic cell cycle Source: ProtInc
Mitotic sister chromatid segregation Source: ProtInc
Mitotic spindle assembly checkpoint signaling Source: ComplexPortal
Mitotic spindle organization Source: UniProtKB
Positive regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Positive regulation of protein localization to kinetochore Source: CAFA
Regulation of protein stability Source: Ensembl
Spindle assembly involved in female meiosis I Source: Ensembl
Cellular Location
Nucleus
Other locations
kinetochore
Note: Localizes to kinetochores from late prophase to anaphase (PubMed:14699129). Localizes specifically to the outer plate of the kinetochore (PubMed:14699129).
PTM
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB.
Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment (PubMed:30409912). Deacetylated by SIRT1 (PubMed:30409912).

Zahm, J. A., Jenni, S., & Harrison, S. C. (2023). Structure of the Ndc80 complex and its interactions at the yeast kinetochore–microtubule interface. Open Biology, 13(3), 220378.

Muir, K. W., Batters, C., Dendooven, T., Yang, J., Zhang, Z., Burt, A., & Barford, D. (2023). Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules. Science, 382(6675), 1184-1190.

Polley, S., Müschenborn, H., Terbeck, M., De Antoni, A., Vetter, I. R., Dogterom, M., ... & Huis in't Veld, P. J. (2023). Stable kinetochore‐microtubule attachment requires loop‐dependent Ndc80‐Ndc80 binding. The EMBO Journal, e112504.

Sarangapani, K. K., Koch, L. B., Nelson, C. R., Asbury, C. L., & Biggins, S. (2021). Kinetochore-bound Mps1 regulates kinetochore–microtubule attachments via Ndc80 phosphorylation. Journal of Cell Biology, 220(12), e202106130.

Dong, Q., Liu, X. L., Wang, X. H., Zhao, Y., Chen, Y. H., & Li, F. (2021). Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly. Proceedings of the National Academy of Sciences, 118(48), e2104459118.

Ustinov, N. B., Korshunova, A. V., & Gudimchuk, N. B. (2020). Protein complex NDC80: Properties, functions, and possible role in pathophysiology of cell division. Biochemistry (Moscow), 85, 448-462.

Wimbish, R. T., & DeLuca, J. G. (2020). Hec1/Ndc80 tail domain function at the kinetochore-microtubule interface. Frontiers in cell and developmental biology, 8, 43.

Wimbish, R. T., DeLuca, K. F., Mick, J. E., Himes, J., Jiménez-Sánchez, I., Jeyaprakash, A. A., & DeLuca, J. G. (2020). The Hec1/Ndc80 tail domain is required for force generation at kinetochores, but is dispensable for kinetochore–microtubule attachment formation and Ska complex recruitment. Molecular Biology of the Cell, 31(14), 1453-1473.

Brusini, L., D’Archivio, S., McDonald, J., & Wickstead, B. (2019). Ndc80/Nuf2-like protein KKIP1 connects a stable kinetoplastid outer kinetochore complex to the inner kinetochore and responds to metaphase tension. BioRxiv, 764829.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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