Rabbit Anti-NDUFS3 Recombinant Antibody (
1H12) (V2LY-0725-LY1067)

Name: Rabbit Anti-NDUFS3 Recombinant Antibody (1H12)
SKU: V2LY-0725-LY1067
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-NDUFS3 Recombinant Antibody (1H12) (V2LY-0725-LY1067) in WB

Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate,
All lanes: NDUFS3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31, 15 KDa
Observed band size: 31 kDa

Rabbit Anti-NDUFS3 Recombinant Antibody (1H12) (V2LY-0725-LY1067) in IHC

IHC image of V2LY-0725-LY1067 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

1H12

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human NDUFS3.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)

Entrez Gene ID

4722

UniProt ID

O75489

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:14729820, PubMed:30140060).

Essential for the catalytic activity and assembly of complex I (PubMed:14729820, PubMed:24028823, PubMed:30140060).

Biological Process

Aerobic respiration Source: ComplexPortal
Mitochondrial ATP synthesis coupled proton transport Source: ComplexPortal
Mitochondrial electron transport, NADH to ubiquinone Source: UniProtKB
Mitochondrial respiratory chain complex I assembly Source: UniProtKB
Negative regulation of cell growth Source: UniProtKB
Negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Reactive oxygen species metabolic process Source: UniProtKB
Substantia nigra development Source: UniProtKB

Cellular Location

Mitochondrion inner membrane

Involvement in disease

Mitochondrial complex I deficiency, nuclear type 8 (MC1DN8):
A form of mitochondrial complex I deficiency, the most common biochemical signature of mitochondrial disorders, a group of highly heterogeneous conditions characterized by defective oxidative phosphorylation, which collectively affects 1 in 5-10000 live births. Clinical disorders have variable severity, ranging from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. MC1DN8 transmission pattern is consistent with autosomal recessive inheritance.

Ma, Y. H., Yang, Y., Li, J. H., Yao, B. C., Chen, Q. L., Wang, L. Q., ... & Guo, S. Z. (2023). NDUFB11 and NDUFS3 regulate arterial atherosclerosis and venous thrombosis: Potential markers of atherosclerosis and venous thrombosis. Medicine, 102(46), e36133.

Kurelac, I., Cavina, B., Sollazzo, M., Miglietta, S., Fornasa, A., De Luise, M., ... & Gasparre, G. (2022). NDUFS3 knockout cancer cells and molecular docking reveal specificity and mode of action of anti-cancer respiratory complex I inhibitors. Open Biology, 12(11), 220198.

D’Angelo, L., Astro, E., De Luise, M., Kurelac, I., Umesh-Ganesh, N., Ding, S., ... & Iommarini, L. (2021). NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Reports, 35(3).

Samanta, D., Veerapandiyan, A., Burrow, T. A., & Gokden, M. (2021). Mitochondrial ultrastructural defects in NDUFS3-related disorder. Journal of Pediatric Neurosciences, 16(4), 299.

D’Angelo, L., Astro, E., De Luise, M., Kurelac, I., Umesh-Ganesh, N., Ding, S., ... & Iommarini, L. (2020). Biogenesis of NDUFS3-less complex I indicates TMEM126A/OPA7 as an assembly factor of the ND4-module. bioRxiv, 2020-10.

Chanthammachat, P., & Dharmasaroja, P. (2019). Metformin restores the mitochondrial membrane potentials in association with a reduction in TIMM23 and NDUFS3 in MPP+-induced neurotoxicity in SH-SY5Y cells. EXCLI journal, 18, 812.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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