Mouse Anti-NEDD9 Recombinant Antibody (1B4) (CBMAB-N0223-WJ)

Name: Mouse Anti-NEDD9 Recombinant Antibody (1B4)
SKU: CBMAB-N0223-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1B4

Application

ELISA, IF, WB

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Neural Precursor Cell Expressed, Developmentally Down-Regulated 9

Introduction

The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Entrez Gene ID

4739

UniProt ID

Q14511

Alternative Names

Neural Precursor Cell Expressed, Developmentally Down-Regulated 9; Cas Scaffolding Protein Family Member 2; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9; Renal Carcinoma Antigen NY-REN-12; CAS-L; CASS2; HEF1; CASL; Crk-Associated Substrate Related Protein Cas-L; CRK-Associated Substrate-Related Protein;

Function

Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. Required for correct adhesion and migration of T-cells (PubMed:17174122).

Biological Process

Actin filament bundle assembly Source: UniProtKB
Actin filament reorganization Source: GO_Central
Activation of GTPase activity Source: Ensembl
Cell adhesion Source: ProtInc
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cell migration Source: GO_Central
Cytoskeleton organization Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of protein tyrosine kinase activity Source: UniProtKB
Positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Regulation of growth Source: UniProtKB-KW
Signal transduction Source: ProtInc
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Cytoplasm
Nucleus
Golgi apparatus
Other locations
cell cortex
lamellipodium
focal adhesion
Note: Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.
Enhancer of filamentation 1 p55:
Cytoskeleton
spindle

PTM

Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected.
PTK2/FAK1 phosphorylates the protein at the YDYVHL motif (conserved among all cas proteins). The SRC family kinases (FYN, SRC, LCK and CRK) are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Ligation of either integrin beta-1 or B-cell antigen receptor on tonsillar B-cells and B-cell lines promotes tyrosine phosphorylation and both integrin and BCR-mediated tyrosine phosphorylation requires an intact actin network. In fibroblasts transformation with oncogene v-ABL results in an increase in tyrosine phosphorylation. Transiently phosphorylated following CD3 cross-linking and this phosphorylated form binds to CRK and C3G. A mutant lacking the SH3 domain is phosphorylated upon CD3 cross-linking but not upon integrin beta-1 cross-linking. Tyrosine phosphorylation occurs upon stimulation of the G-protein coupled C1a calcitonin receptor. Calcitonin-stimulated tyrosine phosphorylation is mediated by calcium- and protein kinase C-dependent mechanisms and requires the integrity of the actin cytoskeleton. Phosphorylation at Ser-369 induces proteasomal degradation.

Hu, Z., Wei, F., Su, Y., Wang, Y., Shen, Y., Fang, Y., ... & Chen, Y. (2023). Histone deacetylase inhibitors promote breast cancer metastasis by elevating NEDD9 expression. Signal Transduction and Targeted Therapy, 8(1), 11.

Deneka, A. Y., Nikonova, A. S., Lee, H. O., Kruger, W. D., & Golemis, E. A. (2022). NEDD9 sustains hexokinase expression to promote glycolysis. Oncogenesis, 11(1), 15.

Deneka, A. Y., Kopp, M. C., Nikonova, A. S., Gaponova, A. V., Kiseleva, A. A., Hensley, H. H., ... & Golemis, E. A. (2021). Nedd9 Restrains Autophagy to Limit Growth of Early Stage Non–Small Cell Lung Cancer. Cancer research, 81(13), 3717-3726.

Alba, G. A., Samokhin, A. O., Wang, R. S., Zhang, Y. Y., Wertheim, B. M., Arons, E., ... & Maron, B. A. (2021). NEDD9 is a novel and modifiable mediator of platelet–endothelial adhesion in the pulmonary circulation. American journal of respiratory and critical care medicine, 203(12), 1533-1545.

Hua, S., Feng, T., Yin, L., Wang, Q., & Shao, X. (2021). NEDD9 overexpression: Prognostic and guidance value in acute myeloid leukaemia. Journal of cellular and molecular medicine, 25(19), 9331-9339.

Samokhin, A. O., Hsu, S., Paul, B. Y., Waxman, A. B., Alba, G. A., Wertheim, B. M., ... & Maron, B. A. (2020). Circulating NEDD9 is increased in pulmonary arterial hypertension: a multicenter, retrospective analysis. The Journal of Heart and Lung Transplantation, 39(4), 289-299.

Meng, H., Wu, J., Huang, Q., Yang, X., Yang, K., Qiu, Y., ... & Qi, H. (2019). NEDD9 promotes invasion and migration of colorectal cancer cell line HCT116 via JNK/EMT. Oncology Letters, 18(4), 4022-4029.

Gu, Y., Lu, J., Chen, C., & Zheng, F. (2019). NEDD9 overexpression predicts poor prognosis in solid cancers: a meta-analysis. OncoTargets and therapy, 12, 4213.

Zhao, S., Min, P., Liu, L., Zhang, L., Zhang, Y., Wang, Y., ... & Gu, L. (2019). NEDD9 facilitates hypoxia-induced gastric cancer cell migration via MICAL1 related Rac1 activation. Frontiers in pharmacology, 10, 291.

Jurčić, P., Radulović, P., Balja, M. P., Milošević, M., & Krušlin, B. (2019). E‑cadherin and NEDD9 expression in primary colorectal cancer, metastatic lymph nodes and liver metastases. Oncology Letters, 17(3), 2881-2889.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

NEDD9 Matched Antibody Pair (784) (CAT#: APMAB-784LY)

NEDD9 Matched Antibody Pair (785) (CAT#: APMAB-785LY)

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For research use only. Not intended for any clinical use.

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