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Mouse Anti-NELFA Recombinant Antibody (6B11H8) (CBMAB-N0001-WJ)

This product is a mouse antibody that recognizes NELFA. The antibody 6B11H8 can be used for immunoassay techniques such as: ELISA, FC, IF, IHC, IHC-P, WB.
See all NELFA antibodies

Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
6B11H8
Antibody Isotype
IgG2b
Application
ELISA, FC, IF, IHC, IHC-P, WB

Basic Information

Immunogen
The immunogen corresponding to a region within aa280-511 of human WHSC2 expressed in E. Coli.
Specificity
Human, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
negative elongation factor complex member A, Whsc2
Introduction
NELFA is a protein-coding gene which is expressed ubiquitously with higher levels in fetal than in adult tissues. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. Diseases associated with NELFA include Wolf-Hirschhorn Syndrome and Chromosomal Deletion Syndrome. Its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Gene Expression. Gene Ontology (GO) annotations related to this gene include chromatin binding and translation elongation factor activity.
Entrez Gene ID
Human7469
Rat305455
UniProt ID
HumanQ9H3P2
RatQ5U2N1
Alternative Names
WHSC2; NELF-A; P/OKcl.15
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.

(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
Biological Process
Multicellular organism development Source: ProtInc
Negative regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
Positive regulation of histone H3-K4 methylation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Cellular Location
Nucleus

Wu, B., Wang, Y., Wei, X., Zhang, J., Wu, J., Cao, G., ... & Bao, S. (2024). NELFA and BCL2 induce the 2C‐like state in mouse embryonic stem cells in a chemically defined medium. Cell Proliferation, 57(2), e13534.

Alikunju, S., Yang, Z., & Sayed, D. H. (2023). Disrupting the NelfA Dependent RNA pol II Paused Complex in Heart Precipitates Dilated Cardiomyopathy. Circulation, 148(Suppl_1), A15512-A15512.

Wang, Y., Na, Q., Li, X., Tee, W. W., Wu, B., & Bao, S. (2021). Retinoic acid induces NELFA‐mediated 2C‐like state of mouse embryonic stem cells associates with epigenetic modifications and metabolic processes in chemically defined media. Cell Proliferation, 54(6), e13049.

Hu, Z., Tan, D. E. K., Chia, G., Tan, H., Leong, H. F., Chen, B. J., ... & Tee, W. W. (2020). Maternal factor NELFA drives a 2C-like state in mouse embryonic stem cells. Nature cell biology, 22(2), 175-186.

Xu, J., Wang, G., Gong, W., Guo, S., Li, D., & Zhan, Q. (2020). The noncoding function of NELFA mRNA promotes the development of oesophageal squamous cell carcinoma by regulating the Rad17‐RFC2‐5 complex. Molecular Oncology, 14(3), 611-624.

Alikunju, S., Severinova, E., Yang, Z., Ivessa, A., & Sayed, D. (2020). Acute NelfA knockdown restricts compensatory gene expression and precipitates ventricular dysfunction during cardiac hypertrophy. Journal of molecular and cellular cardiology, 142, 93-104.

Alikunju, S., Severivona, E., Yang, Z., & Sayed, D. H. (2019). Negative Elongation Factor A (Nelfa) Required for RNA Pol II-Dependent Gene Transcription During Cardiac Hypertrophy. Circulation, 140(Suppl_1), A12992-A12992.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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