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Mouse Anti-NELFA Recombinant Antibody (CBT4361) (V2LY-0625-LY982)


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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBT4361
Antibody Isotype
IgG2b
Application
WB, IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human WHSC2 (AA: 280-511) expressed in E. Coli.
Host Species
Mouse
Specificity
Human, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
negative elongation factor complex member A, Whsc2
Entrez Gene ID
7469
UniProt ID
Q9H3P2
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.

(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
Biological Process
Multicellular organism development Source: ProtInc
Negative regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
Positive regulation of histone H3-K4 methylation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Cellular Location
Nucleus
More Infomation

Wu, B., Wang, Y., Wei, X., Zhang, J., Wu, J., Cao, G., ... & Bao, S. (2024). NELFA and BCL2 induce the 2C‐like state in mouse embryonic stem cells in a chemically defined medium. Cell Proliferation, 57(2), e13534.

Alikunju, S., Yang, Z., & Sayed, D. H. (2023). Disrupting the NelfA Dependent RNA pol II Paused Complex in Heart Precipitates Dilated Cardiomyopathy. Circulation, 148(Suppl_1), A15512-A15512.

Wang, Y., Na, Q., Li, X., Tee, W. W., Wu, B., & Bao, S. (2021). Retinoic acid induces NELFA‐mediated 2C‐like state of mouse embryonic stem cells associates with epigenetic modifications and metabolic processes in chemically defined media. Cell Proliferation, 54(6), e13049.

Hu, Z., Tan, D. E. K., Chia, G., Tan, H., Leong, H. F., Chen, B. J., ... & Tee, W. W. (2020). Maternal factor NELFA drives a 2C-like state in mouse embryonic stem cells. Nature cell biology, 22(2), 175-186.

Xu, J., Wang, G., Gong, W., Guo, S., Li, D., & Zhan, Q. (2020). The noncoding function of NELFA mRNA promotes the development of oesophageal squamous cell carcinoma by regulating the Rad17‐RFC2‐5 complex. Molecular Oncology, 14(3), 611-624.

Alikunju, S., Severinova, E., Yang, Z., Ivessa, A., & Sayed, D. (2020). Acute NelfA knockdown restricts compensatory gene expression and precipitates ventricular dysfunction during cardiac hypertrophy. Journal of molecular and cellular cardiology, 142, 93-104.

Alikunju, S., Severivona, E., Yang, Z., & Sayed, D. H. (2019). Negative Elongation Factor A (Nelfa) Required for RNA Pol II-Dependent Gene Transcription During Cardiac Hypertrophy. Circulation, 140(Suppl_1), A12992-A12992.

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For research use only. Not intended for any clinical use.

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