Sign in or Register   Sign in or Register
  |  

Rabbit Anti-NELFE Recombinant Antibody (CBWJN-0972) (CBMAB-N0249-WJ)

This product is a Rabbit antibody that recognizes NELFE. The antibody CBWJN-0972 can be used for immunoassay techniques such as: WB, IP, ICC.
See all NELFE antibodies

Summary

Host Animal
Rabbit
Specificity
Mouse, Rat, Human
Clone
CBWJN-0972
Antibody Isotype
IgG
Application
WB, IP, ICC

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Negative Elongation Factor Complex Member E
Introduction
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human7936
Mouse27632
Rat294258
UniProt ID
HumanP18615
MouseP19426
RatQ6MG75
Alternative Names
Negative Elongation Factor Complex Member E; RD RNA-Binding Protein; RNA-Binding Protein RD; NELF-E; RDBP; RD; Major Histocompatibility Complex Gene RD;
Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401).

The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062).

Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27282391).

(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
Biological Process
Negative regulation of mRNA polyadenylation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Negative regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Positive regulation of histone H3-K4 methylation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Cellular Location
Nucleus
Other locations
Chromosome
Note: Localizes to chromatin (PubMed:14701750). Phosphorylation by the P-TEFb complex promotes its release from chromatin (PubMed:14701750).
PTM
Phosphorylated by the P-TEFb complex at sites next to its RNA recognition motif, promoting its release from chromatin.
Sumoylated.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-NELFE Recombinant Antibody (CBWJN-0972)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare