Rabbit Anti-NELFE Recombinant Antibody (CBWJN-0972) (CBMAB-N0249-WJ)

Name: Rabbit Anti-NELFE Recombinant Antibody (CBWJN-0972)
SKU: CBMAB-N0249-WJ
Rating: 5 (1 reviews)

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Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBWJN-0972

Application

WB, IP, ICC

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Negative Elongation Factor Complex Member E

Introduction

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	7936
Mouse	27632
Rat	294258

UniProt ID

Human	P18615
Mouse	P19426
Rat	Q6MG75

Alternative Names

Negative Elongation Factor Complex Member E; RD RNA-Binding Protein; RNA-Binding Protein RD; NELF-E; RDBP; RD; Major Histocompatibility Complex Gene RD;

Function

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401).

The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062).

Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27282391).

(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

Biological Process

Negative regulation of mRNA polyadenylation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Negative regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Positive regulation of histone H3-K4 methylation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl

Cellular Location

Nucleus
Other locations
Chromosome
Note: Localizes to chromatin (PubMed:14701750). Phosphorylation by the P-TEFb complex promotes its release from chromatin (PubMed:14701750).

PTM

Phosphorylated by the P-TEFb complex at sites next to its RNA recognition motif, promoting its release from chromatin.
Sumoylated.

Zhang, K., Barry, A., Lamm, R., & Dang, H. (2022). NELFE regulates chromatin accessibility to affect MYC induced transcription. Cancer Research, 82(12_Supplement), 1457-1457.

Yu, S., Li, L., Cai, H., He, B., Gao, Y., & Li, Y. (2021). Overexpression of NELFE contributes to gastric cancer progression via Wnt/β-catenin signaling-mediated activation of CSNK2B expression. Journal of Experimental & Clinical Cancer Research, 40, 1-16.

Chen, C., Zheng, Q., Pan, S., Chen, W., Huang, J., Cao, Y., ... & Jie, Z. (2021). The RNA-binding protein NELFE promotes gastric cancer growth and metastasis through E2F2. Frontiers in oncology, 11, 677111.

Zhang, K., Dang, H., & Barry, A. (2020). NELFE modulates chromatin accessibility to amplify the transcription of MYC-associated genes in hepatocellular carcinoma. Cancer Research, 80(16_Supplement), 2483-2483.

Dang, H., Pomyen, Y., Martin, S. P., Dominguez, D. A., Yim, S. Y., Lee, J. S., ... & Wang, X. W. (2019). NELFE-dependent MYC signature identifies a unique cancer subtype in hepatocellular carcinoma. Scientific reports, 9(1), 3369.

Han, L., Zan, Y., Huang, C., & Zhang, S. (2019). NELFE promoted pancreatic cancer metastasis and the epithelial‑to‑mesenchymal transition by decreasing the stabilization of NDRG2 mRNA. International Journal of Oncology, 55(6), 1313-1323.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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