Mouse Anti-NES Recombinant Antibody (CBWJN-0335) (CBMAB-N0260-WJ)

Name: Mouse Anti-NES Recombinant Antibody (CBWJN-0335)
SKU: CBMAB-N0260-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBWJN-0335

Application

WB, ICC, FC, IHC-P, IP

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nestin

Introduction

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

Entrez Gene ID

10763

UniProt ID

P48681

Alternative Names

Nestin; Nbla00170;

Function

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

Biological Process

Brain development Source: UniProtKB
Cell projection morphogenesis Source: Ensembl
Central nervous system development Source: UniProtKB
Embryonic camera-type eye development Source: UniProtKB
G2/M transition of mitotic cell cycle Source: DFLAT
Negative regulation of catalytic activity Source: UniProtKB
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of protein binding Source: UniProtKB
Positive regulation of intermediate filament depolymerization Source: UniProtKB
Positive regulation of neural precursor cell proliferation Source: UniProtKB
Stem cell proliferation Source: DFLAT

Cellular Location

Cytoskeleton
intermediate filament
intermediate filament cytoskeleton
Other locations
cytoplasm

PTM

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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