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Mouse Anti-Nestin Recombinant Antibody (CBMAB-0438-LY)

This product is mouse antibody recognizes nestin of rat. The antibody immunoassay techniques such as: IF, IHC, IP, WB.
See all Nestin antibodies

Summary

Host Animal
Mouse
Specificity
Rat, Avian, Cat, Human, Mouse
Antibody Isotype
IgG1
Application
IF, IHC, IP, WB

Basic Information

Specificity
Rat, Avian, Cat, Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Nestin
Introduction
Intermediate filament protein specifically expressed in neuroepithelial stem cells. It may plays a role in neural cell differentiation. Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Entrez Gene ID
Human10763
Mouse18008
Rat25491
Cat101083140
UniProt ID
HumanP48681
MouseQ6P5H2
RatP21263
CatA0A5F5XSQ1
Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Biological Process
Brain development Source: UniProtKB
Cell projection morphogenesis Source: Ensembl
Central nervous system development Source: UniProtKB
Embryonic camera-type eye development Source: UniProtKB
G2/M transition of mitotic cell cycle Source: DFLAT
Negative regulation of catalytic activity Source: UniProtKB
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of protein binding Source: UniProtKB
Positive regulation of intermediate filament depolymerization Source: UniProtKB
Positive regulation of neural precursor cell proliferation Source: UniProtKB
Stem cell proliferation Source: DFLAT
Cellular Location
Cytoskeleton
intermediate filament
intermediate filament cytoskeleton
Other locations
cytoplasm
PTM
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).

Calderaro, J., Di Tommaso, L., Maillé, P., Beaufrère, A., Nguyen, C. T., Heij, L., ... & Caruso, S. (2022). Nestin as a diagnostic and prognostic marker for combined hepatocellular-cholangiocarcinoma. Journal of hepatology, 77(6), 1586-1597.

Nishie, H., Nakano-Doi, A., Sawano, T., & Nakagomi, T. (2021). Establishment of a reproducible ischemic stroke model in Nestin-GFP mice with high survival rates. International Journal of Molecular Sciences, 22(23), 12997.

Jaramillo-Rangel, G., Chávez-Briones, M. D. L., Ancer-Arellano, A., & Ortega-Martínez, M. (2021). Nestin-expressing cells in the lung: the bad and the good parts. Cells, 10(12), 3413.

Szymańska-Chabowska, A., Świątkowski, F., Jankowska-Polańska, B., Mazur, G., & Chabowski, M. (2021). Nestin expression as a diagnostic and prognostic marker in colorectal cancer and other tumors. Clinical Medicine Insights: Oncology, 15, 11795549211038256.

Zhang, X., Xing, C., Guan, W., Chen, L., Guo, K., Yu, A., & Xie, K. (2020). Clinicopathological and prognostic significance of nestin expression in patients with breast cancer: a systematic review and meta-analysis. Cancer cell international, 20, 1-17.

Sone, K., Maeno, K., Masaki, A., Kunii, E., Takakuwa, O., Kagawa, Y., ... & Niimi, A. (2020). Nestin expression affects resistance to chemotherapy and clinical outcome in small cell lung cancer. Frontiers in Oncology, 10, 1367.

Wang, J., Lu, Q., Cai, J., Wang, Y., Lai, X., Qiu, Y., ... & Peng Xiang, A. (2019). Nestin regulates cellular redox homeostasis in lung cancer through the Keap1–Nrf2 feedback loop. Nature Communications, 10(1), 5043.

Yamagishi, A., Susaki, M., Takano, Y., Mizusawa, M., Mishima, M., Iijima, M., ... & Nakamura, C. (2019). The structural function of nestin in cell body softening is correlated with cancer cell metastasis. International Journal of Biological Sciences, 15(7), 1546.

Sharma, P., Alsharif, S., Fallatah, A., & Chung, B. M. (2019). Intermediate filaments as effectors of cancer development and metastasis: a focus on keratins, vimentin, and nestin. Cells, 8(5), 497.

Bott, C. J., Johnson, C. G., Yap, C. C., Dwyer, N. D., Litwa, K. A., & Winckler, B. (2019). Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity. Molecular biology of the cell, 30(10), 1214-1229.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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