Nestin Antibodies

Structure of Nestin

Nestin is a type of intermediate filament protein of Class VI with a relatively large molecular weight, approximately 177 to 220 kDa. The specific value may vary slightly depending on the species and post-translational modifications.

This protein is composed of over 1,600 amino acids, and its primary structure contains a highly conserved α -helical rod-shaped core domain, as well as species-specific N-terminal and C-terminal non-helical tails. Nestin assembles into cytoskeletal networks by forming heterodimers (usually paired with Vimentin), and this assembly ability mainly depends on its conserved helical rod-shaped regions. Its C-terminal tail contains multiple phosphorylation sites, which can dynamically regulate the assembly and dissociation of filamentous structures, thereby affecting the mechanical stability and migration ability of the cell.

Fig. 1 Nestin's structure consists of a central α-helical rod domain (blue) flanked by globular NH (green) and COOH (red) domains.¹

Key structural properties of Nestin:

• Characteristic class VI intermediate filament protein α-helical rod domain
• Highly conserved helical regions mediate protein dimerization and filamentous assembly
• The non-helical tail contains multiple phosphorylation sites that regulate the dynamic remodeling of the wire mesh

Functions of Nestin

The protein encoded by the Nestin gene mainly functions as a key component of the cytoskeleton, participating in maintaining cell morphology and supporting cell division. In addition, it is also widely involved in a variety of important physiological and developmental processes, including cell differentiation regulation and tissue regeneration.

The expression of Nestin is highly specific to cell type and developmental stage. Its regulation depends on enhancer-promoter interaction and the activation of signaling pathways (such as Notch and TGF-β), playing an important role in tissue homeostasis and regeneration.

Applications of Nestin and Nestin Antibody in Literature

1. Yao, Senyu, et al. "Nestin-dependent mitochondria-ER contacts define stem Leydig cell differentiation to attenuate male reproductive ageing." Nature communications 13.1 (2022): 4020. https://doi.org/10.1038/s41467-022-31755-w

This article finds that with the increase of age, the differentiation ability of stem cell Lylae's cells (SLC) declines, and the mechanism is related to the reduction of mitochondrial-endoplasmic reticulum contact (MERCs) caused by the absence of the intermediate filament protein Nestin. Melatonin can delay the aging of the male reproductive system by restoring Nestin-dependent MERCs, improving the differentiation potential of SLCS.

2. Jaramillo-Rangel, Gilberto, et al. "Nestin-expressing cells in the lung: the bad and the good parts." Cells 10.12 (2021): 3413. https://doi.org/10.3390/cells10123413

This article indicates that Nestin, as an intermediate filament protein, is highly expressed in lung cancer and is associated with cell proliferation, angiogenesis and metastasis, leading to invasive phenotypes and treatment resistance. Its role in non-cancerous lung diseases and its value as a prognostic marker or therapeutic target still require in-depth research.

3. Bernal, Aurora, and Lorena Arranz. "Nestin-expressing progenitor cells: function, identity and therapeutic implications." Cellular and Molecular Life Sciences 75.12 (2018): 2177-2195. https://doi.org/10.1007/s00018-018-2794-z

This article indicates that Nestin is an intermediate filament protein of the cytoskeleton, widely expressed in stem cells of tissues such as nerves and bone marrow, and participates in regulating their self-renewal, differentiation and migration. Its potential as therapeutic targets and molecular mechanisms in disease models still need to be further explored.

4. Lu, Chunjuan, and Wei. "[Retracted] Nestin Regulates Keap1‐Nrf2‐HO‐1‐Mediated Antioxidant Responses during Stress and Malignant Hematopoiesis." BioMed Research International 2022.1 (2022): 1706585. https://doi.org/10.1155/2022/1706585

This study found that in leukemia patients, Nestin regulates the upregulation of the Keap1-Nrf2-HO-1 signaling pathway, promotes antioxidant responses, and is closely related to the occurrence and development of leukemia, suggesting that Nestin may become a tumor marker.

5. Han, Shunshun, et al. "Nestin Regulates Autophagy‐Dependent Ferroptosis Mediated Skeletal Muscle Atrophy by Ubiquitinating MAP 1LC3B." Journal of Cachexia, Sarcopenia and Muscle 16.2 (2025): e13779. https://doi.org/10.1002/jcsm.13779

Research has found that Nestin alleviates skeletal muscle atrophy by catalyzing the polyubiquitination of the K51 site of the LC3B protein, inhibiting autophagic flux and ferroptosis. The absence of Nestin can exacerbate atrophy. As a key regulatory factor of ferroptosis - autophagy interaction, it provides a new target for the treatment of muscle atrophy.

Creative Biolabs: Nestin Antibodies for Research

Creative Biolabs specializes in the production of high-quality Nestin antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom Nestin Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our Nestin antibodies, custom preparations, or technical support, contact us at email.