Mouse Anti-NET1 Recombinant Antibody (CBWJN-0315) (CBMAB-N0266-WJ)

Name: Mouse Anti-NET1 Recombinant Antibody (CBWJN-0315)
SKU: CBMAB-N0266-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-0315

Application

Dot, WB

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2, 1% BSA

Preservative

0.05% sodium azide

Concentration

0.1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Neuroepithelial Cell Transforming 1

Introduction

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Entrez Gene ID

10276

UniProt ID

Q7Z628

Alternative Names

Neuroepithelial Cell Transforming 1; ARHGEF8; Guanine Nucleotide Regulatory Protein (Oncogene); Neuroepithelial Cell-Transforming Gene 1 Protein; Rho Guanine Nucleotide Exchange Factor (GEF) 8; Rho Guanine Nucleotide Exchange Factor 8;

Function

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Biological Process

Cellular response to hydrogen peroxide Source: UniProtKB
Cellular response to ionizing radiation Source: UniProtKB
Intracellular signal transduction Source: InterPro
Myoblast migration Source: Ensembl
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of GTPase activity Source: UniProtKB
Positive regulation of Rho protein signal transduction Source: GO_Central
Regulation of cell growth Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Signal transduction Source: ProtInc

Cellular Location

Nucleus
Cytoplasm

Li, T., Xiong, X., Wang, Y., Li, Y., Liu, Y., Zhang, M., ... & Shan, H. (2023). Neuroepithelial cell-transforming 1 promotes cardiac fibrosis via the Wnt/β-catenin signaling pathway. Iscience, 26(10), 107888.

Yang, X., Li, Y., Zhang, Y., & Liu, J. (2022). Circ_0000745 promotes acute lymphoblastic leukemia progression through mediating miR-494-3p/NET1 axis. Hematology, 27(1), 11-22.

Ulu, A., Oh, W., Zuo, Y., & Frost, J. A. (2021). Cdk1 phosphorylation negatively regulates the activity of Net1 towards RhoA during mitosis. Cellular signalling, 80, 109926.

Zhao, F., Zhao, Z., Han, Y., Li, S., Liu, C., & Jia, K. (2021). Baicalin suppresses lung cancer growth phenotypes via miR-340-5p/NET1 axis. Bioengineered, 12(1), 1699-1707.

Chen, Z. H., Ni, Q. Z., Zhang, X. P., Ma, N., Feng, J. K., Wang, K., ... & Cheng, S. Q. (2021). NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway. Aging (Albany NY), 13(7), 10672.

Ding, S., Huang, H., Xu, Y., Shen, L., Zhong, C., & Zheng, S. (2020). Neuroepithelial cell transforming gene 1 acts as an oncogene and is mediated by miR-22 in human non-small-cell lung cancer. BioMed Research International, 2020.

Sun, X., Wang, M., Zhang, F., & Kong, X. (2019). Inhibition of NET‑1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway. Experimental and Therapeutic Medicine, 17(3), 2334-2340.

Sun, H., Zhang, Z., Luo, W., Liu, J., Lou, Y., & Xia, S. (2019). NET1 enhances proliferation and chemoresistance in acute lymphoblastic leukemia cells. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, 27(8), 935-944.

Argano, M., De Maria, R., Vogl, C., Rodlsberger, K., Buracco, P., & Menzies, M. P. L. (2019). Canine mammary tumour cells exposure to sevoflurane: effects on cell proliferation and neuroepithelial transforming gene 1 expression. Veterinary anaesthesia and analgesia, 46(3), 369-374.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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