Mouse Anti-NFAM1 Recombinant Antibody (2E9) (CBMAB-N2141-WJ)

This product is a Mouse antibody that recognizes NFAM1. The antibody 2E9 can be used for immunoassay techniques such as: ELISA, WB.
See all NFAM1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2E9

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

NFAT activating protein with ITAM motif 1

Introduction

The protein encoded by this gene is a type I membrane receptor that activates cytokine gene promoters such as the IL-13 and TNF-alpha promoters. The encoded protein contains an immunoreceptor tyrosine-based activation motif (ITAM) and is thought to regulate the signaling and development of B-cells. [provided by RefSeq, Jul 2008]

Entrez Gene ID

150372

UniProt ID

Q8NET5

Alternative Names

NFAT Activating Protein With ITAM Motif 1; Calcineurin/NFAT-Activating ITAM-Containing Protein; CNAIP; NFAT-Activating Protein With ITAM Motif 1; NFAT Activation Molecule 1;

Function

May function in immune system as a receptor which activates via the calcineurin/NFAT-signaling pathway the downstream cytokine gene promoters. Activates the transcription of IL-13 and TNF-alpha promoters. May be involved in the regulation of B-cell, but not T-cell, development. Overexpression activates downstream effectors without ligand binding or antibody cross-linking.

Biological Process

B cell differentiation Source: HGNC-UCL
B cell receptor signaling pathway Source: GO_Central
Inflammatory response Source: HGNC-UCL
Intracellular signal transduction Source: HGNC-UCL
Positive regulation of B cell receptor signaling pathway Source: HGNC-UCL
Positive regulation of cytokine production Source: HGNC-UCL
Positive regulation of DNA-binding transcription factor activity Source: HGNC-UCL
Regulation of B cell differentiation Source: InterPro
Signal transduction Source: HGNC-UCL

Cellular Location

Plasma membrane
Cell membrane
Note: Partially recruited to lipid rafts upon BCR stimulation.

Topology

Extracellular: 43-163
Helical: 164-184
Cytoplasmic: 185-270

PTM

N-glycosylated.

References

Juchem, K. W., Gounder, A. P., Gao, J. P., Seccareccia, E., Yeddula, N., Huffmaster, N. J., ... & Chanda, S. K. (2022). NFAM1 Promotes Pro-Inflammatory Cytokine Production in Mouse and Human Monocytes. Frontiers in Immunology, 12, 773445.

Zhu, J., Chen, Z., Peng, X., Zheng, Z., Le, A., Guo, J., ... & Wang, Q. (2022). Extracellular Vesicle-Derived circITGB1 Regulates Dendritic Cell Maturation and Cardiac Inflammation via miR-342-3p/NFAM1. Oxidative Medicine and Cellular Longevity, 2022.

Ethiraj, P., Haque, I. A., Alford, A. K., Gou, W., Singh, T., Sambandam, Y., ... & Reddy, S. V. (2021). Inhibition of NFAM1 suppresses phospho‐SAPK/JNK signaling during osteoclast differentiation and bone resorption. Journal of Cellular Biochemistry, 122(10), 1534-1543.

Long, J., Chen, J., Wang, Q., Gao, F., Lian, M., Zhang, P., ... & Zhu, H. (2020). NFAT activating protein with ITAM motif 1 (NFAM1) is upregulated on circulating monocytes in coronary artery disease and potentially correlated with monocyte chemotaxis. Atherosclerosis, 307, 39-51.

Zhu, J., Chen, Z., Wang, Q., Guo, J., Ma, L., Shi, H., ... & Zheng, Z. (2020). Exosome-derived circITGB1 regulates dendritic cell maturation and cardiac inflammation via miR-342-3p/NFAM1.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NFAM1 Recombinant Antibody (CBWJN-1353)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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