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Mouse Anti-NFAT5 Recombinant Antibody (1H16) (CBMAB-A5921-LY)

The product is antibody recognizes NFAT5. The antibody 1H16 immunoassay techniques such as: WB, ELISA.
See all NFAT5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1H16
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
NFAT5 (NP_006590.1, 1422 a.a. ~ 1531 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
nuclear factor of activated T-cells 5, tonicity-responsive
Introduction
The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
10725
UniProt ID
O94916
Alternative Names
KIAA0827; NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
Function
Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity (PubMed:10051678).

Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed:22266867).

Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394).
Biological Process
Calcineurin-NFAT signaling cascade Source: GO_Central
Cellular hyperosmotic response Source: ProtInc
Cellular response to cytokine stimulus Source: ARUK-UCL
Positive regulation of gene expression Source: Ensembl
Positive regulation of leukocyte adhesion to vascular endothelial cell Source: ARUK-UCL
Positive regulation of NIK/NF-kappaB signaling Source: ARUK-UCL
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of calcineurin-NFAT signaling cascade Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc
Transcription by RNA polymerase II Source: ProtInc
Cellular Location
Nucleus
Cytoplasm
Note: Nuclear distribution increases under hypertonic conditions.
PTM
Phosphorylated (PubMed:10377394). Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization (PubMed:21209322).

Tillé, L., Cropp, D., Charmoy, M., Reichenbach, P., Andreatta, M., Wyss, T., ... & Verdeil, G. (2023). Activation of the transcription factor NFAT5 in the tumor microenvironment enforces CD8+ T cell exhaustion. Nature Immunology, 24(10), 1645-1653.

Yoshimoto, S., Morita, H., Matsuda, M., Katakura, Y., Hirata, M., & Hashimoto, S. (2021). NFAT5 promotes oral squamous cell carcinoma progression in a hyperosmotic environment. Laboratory Investigation, 101(1), 38-50.

Kumar, R., DuMond, J. F., Khan, S. H., Thompson, E. B., He, Y., Burg, M. B., & Ferraris, J. D. (2020). NFAT5, which protects against hypertonicity, is activated by that stress via structuring of its intrinsically disordered domain. Proceedings of the National Academy of Sciences, 117(33), 20292-20297.

Cen, L., Xing, F., Xu, L., & Cao, Y. (2020). Potential role of gene regulator NFAT5 in the pathogenesis of diabetes mellitus. Journal of Diabetes Research, 2020.

Aramburu, J., & López-Rodríguez, C. (2019). Regulation of inflammatory functions of macrophages and T lymphocytes by NFAT5. Frontiers in immunology, 10, 535.

Lee, N., Kim, D., & Kim, W. U. (2019). Role of NFAT5 in the immune system and pathogenesis of autoimmune diseases. Frontiers in Immunology, 10, 270.

Huerga Encabo, H., Traveset, L., Argilaguet, J., Angulo, A., Nistal-Villán, E., Jaiswal, R., ... & López-Rodríguez, C. (2019). The transcription factor NFAT5 limits infection-induced type I interferon responses. Journal of Experimental Medicine, 217(3), e20190449.

Zhang, S., Liao, K., Miao, Z., Wang, Q., Miao, Y., Guo, Z., ... & Qiu, Y. (2019). CircFOXO3 promotes glioblastoma progression by acting as a competing endogenous RNA for NFAT5. Neuro-oncology, 21(10), 1284-1296.

Jiang, Y., He, R., Jiang, Y., Liu, D., Tao, L., Yang, M., ... & Sun, Y. (2019). Transcription factor NFAT5 contributes to the glycolytic phenotype rewiring and pancreatic cancer progression via transcription of PGK1. Cell death & disease, 10(12), 948.

Ma, P., Zha, S., Shen, X., Zhao, Y., Li, L., Yang, L., ... & Liu, W. (2019). NFAT5 mediates hypertonic stress-induced atherosclerosis via activating NLRP3 inflammasome in endothelium. Cell Communication and Signaling, 17, 1-13.

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For research use only. Not intended for any clinical use.

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