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Mouse Anti-NGEF Recombinant Antibody (6E7) (CBMAB-N2345-WJ)

This product is a Mouse antibody that recognizes NGEF. The antibody 6E7 can be used for immunoassay techniques such as: ELISA, WB.
See all NGEF antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
6E7
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
neuronal guanine nucleotide exchange factor
Introduction
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
Entrez Gene ID
25791
UniProt ID
Q8N5V2
Alternative Names
Neuronal Guanine Nucleotide Exchange Factor; Eph-Interacting Exchange Protein; Ephexin; Ephexin-1; ARHGEF27;
Function
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
Biological Process
Activation of GTPase activityManual Assertion Based On ExperimentIBA:GO_Central
Cell differentiationIEA:UniProtKB-KW
Ephrin receptor signaling pathwayISS:UniProtKB
Negative regulation of dendritic spine morphogenesisISS:UniProtKB
Nervous system developmentIEA:UniProtKB-KW
Regulation of GTPase activityISS:UniProtKB
Regulation of small GTPase mediated signal transductionTAS:Reactome
Cellular Location
Activation of GTPase activityManual Assertion Based On ExperimentIBA:GO_Central
Cell differentiationIEA:UniProtKB-KW
Ephrin receptor signaling pathwayISS:UniProtKB
Negative regulation of dendritic spine morphogenesisISS:UniProtKB
Nervous system developmentIEA:UniProtKB-KW
Regulation of GTPase activityISS:UniProtKB
Regulation of small GTPase mediated signal transductionTAS:Reactome
PTM
Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. Phosphorylation by CDK5 upon EPHA4 activation by EFNA1 may regulate dendritic spine morphogenesis (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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