Mouse Anti-NGEF Recombinant Antibody (6E7) (CBMAB-N2345-WJ)

Name: Mouse Anti-NGEF Recombinant Antibody (6E7)
SKU: CBMAB-N2345-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6E7

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

neuronal guanine nucleotide exchange factor

Introduction

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

Entrez Gene ID

25791

UniProt ID

Q8N5V2

Alternative Names

Neuronal Guanine Nucleotide Exchange Factor; Eph-Interacting Exchange Protein; Ephexin; Ephexin-1; ARHGEF27;

Function

Biological Process

Activation of GTPase activityManual Assertion Based On ExperimentIBA:GO_Central
Cell differentiationIEA:UniProtKB-KW
Ephrin receptor signaling pathwayISS:UniProtKB
Negative regulation of dendritic spine morphogenesisISS:UniProtKB
Nervous system developmentIEA:UniProtKB-KW
Regulation of GTPase activityISS:UniProtKB
Regulation of small GTPase mediated signal transductionTAS:Reactome

Cellular Location

PTM

Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. Phosphorylation by CDK5 upon EPHA4 activation by EFNA1 may regulate dendritic spine morphogenesis (By similarity).

Fu, Z., Wang, Y., Wang, C., Yu, Z., Huang, W., Zheng, K., ... & Chen, J. (2023). Up-regulation of NGEF by ERK/AP1 signaling pathway in BRAFV600E-mutant thyroid cancer promotes cancer metastasis.

Sasaki, K., Arimoto, K., Kankawa, K., Terada, C., Yamamori, T., Watakabe, A., & Yamamoto, N. (2020). Rho guanine nucleotide exchange factors regulate horizontal axon branching of cortical upper layer neurons. Cerebral Cortex, 30(4), 2506-2518.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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