Rabbit Anti-NKD2 Recombinant Antibody (S.902.7) (CBMAB-N2532-WJ)

This product is a Rabbit antibody that recognizes NKD2. The antibody S.902.7 can be used for immunoassay techniques such as: IP, WB.
See all NKD2 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human

Clone

S.902.7

Antibody Isotype

IgG

Application

IP, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.01M HEPES, 0.15M NaCl, BSA, 50% glycerol, pH 7.5

Preservative

0.02% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Naked Cuticle Homolog 2

Introduction

This gene encodes a member of a family of proteins that function as negative regulators of Wnt receptor signaling through interaction with Dishevelled family members. The encoded protein participates in the delivery of transforming growth factor alpha-containing vesicles to the cell membrane. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Entrez Gene ID

85409

UniProt ID

Q969F2

Alternative Names

Naked Cuticle Homolog 2; Dvl-Binding Protein NKD2; Naked Cuticle Homolog 2 (Drosophila); Protein Naked Cuticle Homolog 2; Naked Cuticle-2; Naked-2; Naked2; HNkd2;

Function

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity).
Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.

Biological Process

ExocytosisIEA:UniProtKB-KW
Golgi vesicle fusion to target membraneManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of Wnt signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of proteasomal ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein localization to plasma membraneManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of protein processingManual Assertion Based On ExperimentIDA:BHF-UCL
Protein localization to plasma membraneManual Assertion Based On ExperimentIMP:BHF-UCL
Wnt signaling pathwayIEA:UniProtKB-KW

Cellular Location

Cell membrane
Cytoplasm
Cytoplasmic vesicle

PTM

Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.

References

Ke, S., Pan, Q., Wang, C., Su, Z., Li, M., & Liu, X. (2023). NKD2 Trigger NF-κB Signaling Pathway and Facilitates Thyroid Cancer Cell Proliferation. Molecular Biotechnology, 1-11.

Bell, I., Khan, H., Stutt, N., Horn, M., Hydzik, T., Lum, W., ... & Van Raay, T. J. (2023). Loss of Nkd1 is dominant over loss of Axin2 in regulating Wnt signaling. bioRxiv, 2023-10.

Gao, Y., Wang, Y., & Guo, R. (2022). NKD2 is correlated with the occurrence, progression and prognosis of thyroid carcinoma. European Journal of Medical Research, 27(1), 235.

Lu, Y., Zhang, R., Zhang, X., Zhang, B., & Yao, Q. (2020). Curcumin may reverse 5-fluorouracil resistance on colonic cancer cells by regulating TET1-NKD-Wnt signal pathway to inhibit the EMT progress. Biomedicine & Pharmacotherapy, 129, 110381.

Gammons, M. V., Renko, M., Flack, J. E., Mieszczanek, J., & Bienz, M. (2020). Feedback control of Wnt signaling based on ultrastable histidine cluster co-aggregation between Naked/NKD and Axin. Elife, 9, e59879.

Gammons, M., Renko, M., Flack, J. E., Mieszczanek, J., & Bienz, M. (2020). Feedback control of Wnt signaling based on histidine cluster co-aggregation between Naked/NKD and Axin. bioRxiv, 2020-06.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Antibody Pairs

NKD2 Matched Antibody Pair (800)

Related Products

Rabbit Anti-NKD2 Recombinant Antibody (C67C4)

NKD2 Matched Antibody Pair (800)

Rabbit Anti-NKD2 Recombinant Antibody (C36D3)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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