Armenian Hamster Anti-NKG2D Recombinant Antibody (HMG2D) (CBMAB-1072-LY)

Name: Armenian Hamster Anti-NKG2D Recombinant Antibody (HMG2D)
SKU: CBMAB-1072-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Armenian Hamster

Clone

HMG2D

Application

Blocking

Specificity

Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

killer cell lectin-like receptor subfamily K, member 1

Introduction

Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha.

Entrez Gene ID

27007

UniProt ID

O54709

Alternative Names

Nkg2d; NKG2-D; D6H12S2489E

Function

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including RAET1A, RAET1B, RAET1C, RAET1D, RAET1E, H60 and MULT1.

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
Cell differentiationIEA:UniProtKB-KW
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIDA:MGI
Defense response to Gram-positive bacteriumManual Assertion Based On ExperimentIDA:MGI
Natural killer cell activationManual Assertion Based On ExperimentIMP:MGI
Natural killer cell mediated cytotoxicityIEA:Ensembl
Negative regulation of GTPase activityIEA:Ensembl
Negative regulation of natural killer cell chemotaxisIEA:Ensembl
Positive regulation of apoptotic processISO:MGI
Positive regulation of interferon-gamma productionManual Assertion Based On ExperimentIDA:MGI
Positive regulation of myeloid dendritic cell activationISO:MGI
Positive regulation of natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell targetISO:MGI
Positive regulation of nitric oxide biosynthetic processManual Assertion Based On ExperimentIDA:MGI
Stimulatory C-type lectin receptor signaling pathwayManual Assertion Based On ExperimentIMP:MGI

Cellular Location

Cell membrane
Colocalized with HCST and TYROBP on the cell surface.

Involvement in disease

Involved in autoreactive CD8(+) T-cell-mediated development of autoimmune diabetes.

Topology

Cytoplasmic: 1-66
Helical: 67-89
Extracellular: 90-232

Tan, G., Spillane, K. M., & Maher, J. (2023). The role and regulation of the NKG2D/NKG2D ligand system in cancer. Biology, 12(8), 1079.

Cadoux, M., Caruso, S., Pham, S., Gougelet, A., Pophillat, C., Riou, R., ... & Couty, J. P. (2021). Expression of NKG2D ligands is downregulated by β-catenin signalling and associates with HCC aggressiveness. Journal of Hepatology, 74(6), 1386-1397.

Leivas, A., Valeri, A., Córdoba, L., García-Ortiz, A., Ortiz, A., Sánchez-Vega, L., ... & Martínez-López, J. (2021). NKG2D-CAR-transduced natural killer cells efficiently target multiple myeloma. Blood Cancer Journal, 11(8), 146.

Fuertes, M. B., Domaica, C. I., & Zwirner, N. W. (2021). Leveraging NKG2D ligands in immuno-oncology. Frontiers in Immunology, 12, 713158.

Curio, S., Jonsson, G., & Marinović, S. (2021). A summary of current NKG2D-based CAR clinical trials. Immunotherapy Advances, 1(1), ltab018.

Lazarova, M., & Steinle, A. (2019). Impairment of NKG2D-mediated tumor immunity by TGF-β. Frontiers in immunology, 10, 2689.

Frazao, A., Rethacker, L., Messaoudene, M., Avril, M. F., Toubert, A., Dulphy, N., & Caignard, A. (2019). NKG2D/NKG2-ligand pathway offers new opportunities in cancer treatment. Frontiers in Immunology, 10, 661.

Liu, H., Wang, S., Xin, J., Wang, J., Yao, C., & Zhang, Z. (2019). Role of NKG2D and its ligands in cancer immunotherapy. American journal of cancer research, 9(10), 2064.

Paczulla, A. M., Rothfelder, K., Raffel, S., Konantz, M., Steinbacher, J., Wang, H., ... & Lengerke, C. (2019). Absence of NKG2D ligands defines leukaemia stem cells and mediates their immune evasion. Nature, 572(7768), 254-259.

Lazarova, M., & Steinle, A. (2019). The NKG2D axis: an emerging target in cancer immunotherapy. Expert opinion on therapeutic targets, 23(4), 281-294.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
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For research use only. Not intended for any clinical use.

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