Mouse Anti-NKRF Recombinant Antibody (CBWJN-0318) (CBMAB-N0391-WJ)

This product is a Mouse antibody that recognizes NKRF. The antibody CBWJN-0318 can be used for immunoassay techniques such as: Dot, WB.
See all NKRF antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBWJN-0318

Antibody Isotype

IgG2b

Application

Dot, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2, 1% BSA

Preservative

0.05% sodium azide

Concentration

0.1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

NFKB repressing factor

Introduction

This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Entrez Gene ID

55922

UniProt ID

O15226

Alternative Names

NFKB Repressing Factor; Transcription Factor NRF; ITBA4; NRF; NF-Kappa-B-Repressing Factor; NF-KappaB Repressing Factor; NFkB-Repressing Factor; Protein ITBA4;

Function

Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15 (PubMed:12381793).
Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity (PubMed:12381793).
Also involved in the regulation of IL-8 transcription (PubMed:12381793).
May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes (PubMed:10562553).

Biological Process

Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB

Cellular Location

Nucleus, nucleolus

References

Wang, W. F., Zhong, H. J., Cheng, S., Fu, D., Zhao, Y., Cai, H. M., ... & Zhao, W. L. (2023). A nuclear NKRF interacting long noncoding RNA controls EBV eradication and suppresses tumor progression in natural killer/T-cell lymphoma. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1869(6), 166722.

Lu, J., Shi, Y., Zhang, F., Zhang, Y., Zhao, X., Zheng, H., ... & Zhao, L. (2023). Mechanism of lnRNA-ICL involved in lung cancer development in COPD patients through modulating microRNA-19-3p/NKRF/NF-κB axis. Cancer Cell International, 23(1), 58.

Zhang, Y., Meng, T., Chen, J., Zhang, Y., Kang, J., Li, X., ... & Ning, B. (2021). miR-21a-5p promotes inflammation following traumatic spinal cord injury through upregulation of neurotoxic reactive astrocyte (A1) polarization by inhibiting the CNTF/STAT3/Nkrf pathway. International Journal of Biological Sciences, 17(11), 2795.

Xu, X., Xia, Y., Ma, J., Li, W., Niu, N., Li, X., ... & He, X. (2020). Upregulation of miRNA‑301a‑3p promotes tumor progression in gastric cancer by suppressing NKRF and activating NF‑κB signaling. International Journal of Oncology, 57(2), 522-532.

Chen, X. J., Zhang, W. N., Chen, B., Xi, W. D., Lu, Y., Huang, J. Y., ... & Chen, S. J. (2019). Homoharringtonine deregulates MYC transcriptional expression by directly binding NF-κB repressing factor. Proceedings of the National Academy of Sciences, 116(6), 2220-2225.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NKRF Recombinant Antibody (1F6)

Mouse Anti-NKRF Recombinant Antibody (4C2)

Mouse Anti-NKRF Recombinant Antibody (CBWJN-0493)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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