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Mouse Anti-NLRC3 Recombinant Antibody (E-4) (CBMAB-N2670-WJ)

This product is a Mouse antibody that recognizes NLRC3. The antibody E-4 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all NLRC3 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
E-4
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human, Mouse, Rat
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NLR Family CARD Domain Containing 3
Introduction
This gene encodes a NOD-like receptor family member. The encoded protein is a cytosolic regulator of innate immunity. This protein directly interacts with stimulator of interferon genes (STING), to prevent its proper trafficking, resulting in disruption of STING-dependent activation of the innate immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Entrez Gene ID
Human197358
Mouse268857
Rat287070
UniProt ID
HumanQ7RTR2
MouseQ5DU56
RatD3ZYP9
Alternative Names
NLR Family CARD Domain Containing 3; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And CARD Domain Containing 3; NACHT, LRR And CARD Domains-Containing Protein 3; Caterpiller Protein 16.2; NOD-Like Receptor C3; CARD15-Like Protein;
Function
Negative regulator of the innate immune response (PubMed:15705585, PubMed:22863753, PubMed:25277106).
Attenuates signaling pathways activated by Toll-like receptors (TLRs) and the DNA sensor STING/TMEM173 in response to pathogen-associated molecular patterns, such as intracellular poly(dA:dT), but not poly(I:C), or in response to DNA virus infection, including that of Herpes simplex virus 1 (HSV1) (By similarity) (PubMed:22863753).
May affect TLR4 signaling by acting at the level of TRAF6 ubiquitination, decreasing the activating 'Lys-63'-linked ubiquitination and leaving unchanged the degradative 'Lys-48'-linked ubiquitination (PubMed:22863753).
Inhibits the PI3K-AKT-mTOR pathway possibly by directly interacting with the posphatidylinositol 3-kinase regulatory subunit p85 (PIK3R1/PIK3R2) and disrupting the association between PIK3R1/PIK3R2 and the catalytic subunit p110 (PIK3CA/PIK3CB/PIK3CD) and reducing PIK3R1/PIK3R2 activation. Via its regulation of the PI3K-AKT-mTOR pathway, controls cell proliferation, predominantly in intestinal epithelial cells (By similarity).
May also affect NOD1- or NOD2-mediated NF-kappa-B activation (PubMed:25277106).
Might also affect the inflammatory response by preventing NLRP3 inflammasome formation, CASP1 cleavage and IL1B maturation (PubMed:25277106).
Biological Process
I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of cytokine production involved in inflammatory responseISS:UniProtKB
Negative regulation of epithelial cell proliferationISS:UniProtKB
Negative regulation of fibroblast proliferationISS:UniProtKB
Negative regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIMP:MGI
Negative regulation of inflammatory responseManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of innate immune responseISS:UniProtKB
Negative regulation of interferon-alpha productionISS:UniProtKB
Negative regulation of interferon-beta productionISS:UniProtKB
Negative regulation of interleukin-6 productionManual Assertion Based On ExperimentIDA:CACAO
Negative regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of NIK/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of NLRP3 inflammasome complex assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of phosphatidylinositol 3-kinase signalingISS:UniProtKB
Negative regulation of tumor necrosis factor productionManual Assertion Based On ExperimentIDA:CACAO
T cell activationManual Assertion Based On ExperimentIEP:UniProtKB
Cellular Location
Cytoplasm

Zhao, Y., & Li, R. (2023). Overview of the anti-inflammatory function of the innate immune sensor NLRC3. Molecular Immunology, 153, 36-41.

Gao, C., Cai, X., Lymbery, A. J., Ma, L., & Li, C. (2023). The evolution of NLRC3 subfamily genes in Sebastidae teleost fishes. BMC genomics, 24(1), 683.

Sun, D., Xu, J., Zhang, W., Song, C., Gao, C., He, Y., & Shang, Y. (2022). Negative regulator NLRC3: Its potential role and regulatory mechanism in immune response and immune-related diseases. Frontiers in Immunology, 13, 1012459.

Qin, Y., Wu, K., Zhang, Z., Pan, R., Lin, Z., Zhang, W., ... & Lu, X. (2022). NLRC3 deficiency promotes cutaneous wound healing due to the inhibition of p53 signaling. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1868(11), 166518.

Chang, M. X., Xiong, F., Wu, X. M., & Hu, Y. W. (2021). The expanding and function of NLRC3 or NLRC3-like in teleost fish: Recent advances and novel insights. Developmental & Comparative Immunology, 114, 103859.

Ma, R., Zhang, X., Shu, J., Liu, Z., Sun, W., Hou, S., ... & Wu, X. (2021). Nlrc3 knockout mice showed renal pathological changes after HTNV infection. Frontiers in immunology, 12, 692509.

Zha, L. H., Zhou, J., Tan, Y., Guo, S., Zhang, M. Q., Li, S., ... & Yu, Z. X. (2020). NLRC3 inhibits PDGF‐induced PASMCs proliferation via PI3K‐mTOR pathway. Journal of cellular physiology, 235(12), 9557-9567.

Wang, T., Yan, B., Lou, L., Lin, X., Yu, T., Wu, S., ... & Wen, Z. (2019). Nlrc3-like is required for microglia maintenance in zebrafish. Journal of Genetics and Genomics, 46(6), 291-299.

Li, X., Deng, M., Petrucelli, A. S., Zhu, C., Mo, J., Zhang, L., ... & Ting, J. P. Y. (2019). Viral DNA binding to NLRC3, an inhibitory nucleic acid sensor, unleashes STING, a cyclic dinucleotide receptor that activates type I interferon. Immunity, 50(3), 591-599.

Fu, Y., Zhan, X., Wang, Y., Jiang, X., Liu, M., Yang, Y., ... & Hu, S. (2019). NLRC 3 expression in dendritic cells attenuates CD 4+ T cell response and autoimmunity. The EMBO Journal, 38(16), e101397.

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For research use only. Not intended for any clinical use.

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