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Rat Anti-NLRC4 Recombinant Antibody (CBYY-C2704) (CBMAB-C4145-YY)

This product is rat antibody that recognizes NLRC4. The antibody CBYY-C2704 can be used for immunoassay techniques such as: ELISA, FC, WB
See all NLRC4 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYY-C2704
Antibody Isotype
IgG1
Application
ELISA, FC, WB

Basic Information

Immunogen
p20 fragment of mouse caspase-12 and synthetic peptide corresponding to aa 183-205 (N183KKFDYLFDRDNADTDILNMQEL205) of mouse caspase-12
Specificity
Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Protein G purified
Buffer
1 mg/mL
Preservative
PBS
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NLR Family CARD Domain Containing 4
Entrez Gene ID
268973
UniProt ID
Q3UP24
Alternative Names
CYPA, CYPH, HEL-S-69p
Function
Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016).
The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).
Biological Process
Activation of cysteine-type endopeptidase activityManual Assertion Based On ExperimentIMP:CACAO
Activation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIDA:HGNC-UCL
Activation of innate immune responseISS:UniProtKB
Apoptotic processIEA:UniProtKB-KW
Defense response to bacteriumManual Assertion Based On ExperimentIDA:HGNC-UCL
Detection of bacteriumManual Assertion Based On ExperimentIDA:HGNC-UCL
Icosanoid biosynthetic process1 PublicationIC:ComplexPortal
Inflammatory responseISS:UniProtKB
Innate immune responseIEA:UniProtKB-KW
Pattern recognition receptor signaling pathway1 PublicationIC:ComplexPortal
Positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of inflammatory response1 PublicationIC:ComplexPortal
Positive regulation of interleukin-1 beta productionManual Assertion Based On ExperimentIGI:HGNC-UCL
Positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein processingManual Assertion Based On ExperimentIGI:ARUK-UCL
Protein homooligomerizationISS:UniProtKB
PyroptosisISS:UniProtKB
Cellular Location
Cytoplasm
Cytoplasm, cytosol
Inflammasome
Involvement in disease
Autoinflammation with infantile enterocolitis (AIFEC):
An autosomal dominant disorder characterized by neonatal-onset enterocolitis, periodic fever, and fatal or near-fatal episodes of autoinflammation. Affected individuals tend to have poor overall growth and gastrointestinal symptoms in infancy, recurrent febrile episodes with splenomegaly, and sometimes hematologic disturbances, arthralgias, or myalgias.
Familial cold autoinflammatory syndrome 4 (FCAS4):
A form of autoinflammatory syndrome, a rare autosomal dominant systemic disease characterized by recurrent episodes of maculopapular rash associated with arthralgias, myalgias, fever and chills, swelling of the extremities, and conjunctivitis after generalized exposure to cold.
PTM
Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro.

Ionescu, D., Peñín‐Franch, A., Mensa‐Vilaró, A., Castillo, P., Hurtado‐Navarro, L., Molina‐López, C., ... & Aróstegui, J. I. (2022). First Description of Late‐Onset Autoinflammatory Disease Due to Somatic NLRC4 Mosaicism. Arthritis & Rheumatology, 74(4), 692-699.

Guan, C., Huang, X., Yue, J., Xiang, H., Shaheen, S., Jiang, Z., ... & Wu, X. (2021). SIRT3-mediated deacetylation of NLRC4 promotes inflammasome activation. Theranostics, 11(8), 3981.

Sundaram, B., & Kanneganti, T. D. (2021). Advances in understanding activation and function of the NLRC4 inflammasome. International journal of molecular sciences, 22(3), 1048.

Wen, J., Xuan, B., Liu, Y., Wang, L., He, L., Meng, X., ... & Wang, Y. (2021). Updating the NLRC4 inflammasome: from bacterial infections to autoimmunity and cancer. Frontiers in Immunology, 12, 702527.

Andrade, W. A., & Zamboni, D. S. (2020). NLRC4 biology in immunity and inflammation. Journal of Leucocyte Biology, 108(4), 1117-1127.

Bauer, R., & Rauch, I. (2020). The NAIP/NLRC4 inflammasome in infection and pathology. Molecular Aspects of Medicine, 76, 100863.

Kay, C., Wang, R., Kirkby, M., & Man, S. M. (2020). Molecular mechanisms activating the NAIP‐NLRC4 inflammasome: implications in infectious disease, autoinflammation, and cancer. Immunological reviews, 297(1), 67-82.

Poh, L., Kang, S. W., Baik, S. H., Ng, G. Y. Q., She, D. T., Balaganapathy, P., ... & Arumugam, T. V. (2019). Evidence that NLRC4 inflammasome mediates apoptotic and pyroptotic microglial death following ischemic stroke. Brain, Behavior, and Immunity, 75, 34-47.

Semper, R. P., Vieth, M., Gerhard, M., & Mejías-Luque, R. (2019). Helicobacter pylori exploits the NLRC4 inflammasome to dampen host defenses. The Journal of Immunology, 203(8), 2183-2193.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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