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Rat Anti-NLRC4 Recombinant Antibody (M549) (CBMAB-N2675-WJ)

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Basic Information

Host Animal
Rat
Clone
M549
Application
WB, ELISA
Specificity
Human, Rat, Mouse
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
NLR Family CARD Domain Containing 4
Introduction
This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Entrez Gene ID
Human58484
Mouse268973
Rat298784
UniProt ID
HumanQ9NPP4
MouseQ3UP24
RatF1M649
Alternative Names
NLR Family CARD Domain Containing 4; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And CARD Domain Containing 4; Caspase Recruitment Domain-Containing Protein 12; Caspase Recruitment Domain Family, Member 12; CARD, LRR, And NACHT-Containing Protein; NOD-Like Receptor C4; CARD12; CLAN1; CLAN; IPAF; NLR Family CARD Domain-Containing Protein 4;
Function
Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016).
The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).
Biological Process
Activation of cysteine-type endopeptidase activityManual Assertion Based On ExperimentIMP:CACAO
Activation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIDA:HGNC-UCL
Activation of innate immune responseISS:UniProtKB
Apoptotic processIEA:UniProtKB-KW
Defense response to bacteriumManual Assertion Based On ExperimentIDA:HGNC-UCL
Detection of bacteriumManual Assertion Based On ExperimentIDA:HGNC-UCL
Icosanoid biosynthetic process1 PublicationIC:ComplexPortal
Inflammatory responseISS:UniProtKB
Innate immune responseIEA:UniProtKB-KW
Pattern recognition receptor signaling pathway1 PublicationIC:ComplexPortal
Positive regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of inflammatory response1 PublicationIC:ComplexPortal
Positive regulation of interleukin-1 beta productionManual Assertion Based On ExperimentIGI:HGNC-UCL
Positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein processingManual Assertion Based On ExperimentIGI:ARUK-UCL
Protein homooligomerizationISS:UniProtKB
PyroptosisISS:UniProtKB
Cellular Location
Cytoplasm
Cytoplasm, cytosol
Inflammasome
Involvement in disease
Autoinflammation with infantile enterocolitis (AIFEC):
An autosomal dominant disorder characterized by neonatal-onset enterocolitis, periodic fever, and fatal or near-fatal episodes of autoinflammation. Affected individuals tend to have poor overall growth and gastrointestinal symptoms in infancy, recurrent febrile episodes with splenomegaly, and sometimes hematologic disturbances, arthralgias, or myalgias.
Familial cold autoinflammatory syndrome 4 (FCAS4):
A form of autoinflammatory syndrome, a rare autosomal dominant systemic disease characterized by recurrent episodes of maculopapular rash associated with arthralgias, myalgias, fever and chills, swelling of the extremities, and conjunctivitis after generalized exposure to cold.
PTM
Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro.

Ionescu, D., Peñín‐Franch, A., Mensa‐Vilaró, A., Castillo, P., Hurtado‐Navarro, L., Molina‐López, C., ... & Aróstegui, J. I. (2022). First Description of Late‐Onset Autoinflammatory Disease Due to Somatic NLRC4 Mosaicism. Arthritis & Rheumatology, 74(4), 692-699.

Guan, C., Huang, X., Yue, J., Xiang, H., Shaheen, S., Jiang, Z., ... & Wu, X. (2021). SIRT3-mediated deacetylation of NLRC4 promotes inflammasome activation. Theranostics, 11(8), 3981.

Sundaram, B., & Kanneganti, T. D. (2021). Advances in understanding activation and function of the NLRC4 inflammasome. International journal of molecular sciences, 22(3), 1048.

Wen, J., Xuan, B., Liu, Y., Wang, L., He, L., Meng, X., ... & Wang, Y. (2021). Updating the NLRC4 inflammasome: from bacterial infections to autoimmunity and cancer. Frontiers in Immunology, 12, 702527.

Andrade, W. A., & Zamboni, D. S. (2020). NLRC4 biology in immunity and inflammation. Journal of Leucocyte Biology, 108(4), 1117-1127.

Bauer, R., & Rauch, I. (2020). The NAIP/NLRC4 inflammasome in infection and pathology. Molecular Aspects of Medicine, 76, 100863.

Kay, C., Wang, R., Kirkby, M., & Man, S. M. (2020). Molecular mechanisms activating the NAIP‐NLRC4 inflammasome: implications in infectious disease, autoinflammation, and cancer. Immunological reviews, 297(1), 67-82.

Poh, L., Kang, S. W., Baik, S. H., Ng, G. Y. Q., She, D. T., Balaganapathy, P., ... & Arumugam, T. V. (2019). Evidence that NLRC4 inflammasome mediates apoptotic and pyroptotic microglial death following ischemic stroke. Brain, Behavior, and Immunity, 75, 34-47.

Semper, R. P., Vieth, M., Gerhard, M., & Mejías-Luque, R. (2019). Helicobacter pylori exploits the NLRC4 inflammasome to dampen host defenses. The Journal of Immunology, 203(8), 2183-2193.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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