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Rabbit Anti-NME2 Recombinant Antibody (CBWJN-1004) (CBMAB-N0429-WJ)

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Basic Information

Host Animal
Rabbit
Clone
CBWJN-1004
Application
WB, IHC-P, FC, ICC
Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
NME2
Introduction
Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]
Entrez Gene ID
Human4831
Mouse18103
Rat83782
UniProt ID
HumanP22392
MouseQ01768
RatP19804
Alternative Names
NME/NM23 Nucleoside Diphosphate Kinase 2; Non-Metastatic Cells 2, Protein (NM23B) Expressed In; C-Myc Purine-Binding Transcription Factor PUF; Histidine Protein Kinase NDKB; NDP Kinase B; EC 2.7.4.6; NM23-H2; NM23B; Epididymis Secretory Sperm Binding Protein Li 155an; Non-Metastatic Cells 2, Protein (NM23) Expressed In; Nucleoside Diphosphate Kinase B;
Function
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity).
Negatively regulates Rho activity by interacting with AKAP13/LBC (PubMed:15249197).
Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752, PubMed:19435876).
Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III1 region of the MYC gene promoter. Does not bind to duplex NHE III1 (PubMed:19435876).
Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041).
Exhibits histidine protein kinase activity (PubMed:20946858).
Biological Process
Cell adhesionManual Assertion Based On ExperimentTAS:HGNC-UCL
CTP biosynthetic processIEA:InterPro
GTP biosynthetic processIEA:InterPro
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:HGNC-UCL
Nucleoside diphosphate phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Nucleoside triphosphate biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of epithelial cell proliferationManual Assertion Based On ExperimentIMP:HGNC-UCL
Positive regulation of keratinocyte differentiationManual Assertion Based On ExperimentIMP:HGNC-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of apoptotic processManual Assertion Based On ExperimentIBA:GO_Central
Regulation of epidermis developmentManual Assertion Based On ExperimentIMP:HGNC-UCL
UTP biosynthetic processIEA:InterPro
Cellular Location
Cytoplasm
Cell projection, lamellipodium
Cell projection, ruffle
Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.
Isoform 1
Cytoplasm
Cytoplasm, perinuclear region
Nucleus
Isoform 3
Cytoplasm
Cytoplasm, perinuclear region
Nucleus

Iuso, D., Garcia-Saez, I., Couté, Y., Yamaryo-Botté, Y., Boeri Erba, E., Adrait, A., ... & Khochbin, S. (2023). Nucleoside diphosphate kinases 1 and 2 regulate a protective liver response to a high-fat diet. Science Advances, 9(36), eadh0140.

Xie, L., Wang, S., Xie, Z., Wang, X., Wan, L., Deng, X., ... & Li, M. (2021). Gallus NME/NM23 nucleoside diphosphate kinase 2 interacts with viral σA and affects the replication of avian reovirus. Veterinary Microbiology, 252, 108926.

Adam, K., Ning, J., Reina, J., & Hunter, T. (2020). NME/NM23/NDPK and histidine phosphorylation. International journal of molecular sciences, 21(16), 5848.

Radić, M., Šoštar, M., Weber, I., Ćetković, H., Slade, N., & Herak Bosnar, M. (2020). The subcellular localization and oligomerization preferences of NME1/NME2 upon radiation-induced DNA damage. International Journal of Molecular Sciences, 21(7), 2363.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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