Mouse Anti-NOBOX Recombinant Antibody (4C5) (CBMAB-N2856-WJ)

This product is a Mouse antibody that recognizes NOBOX. The antibody 4C5 can be used for immunoassay techniques such as: WB, IHC .
See all NOBOX antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

4C5

Antibody Isotype

IgG1

Application

WB, IHC

Basic Information

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1.00 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

NOBOX Oogenesis Homeobox

Introduction

This homeobox gene encodes a transcription factor that is thought to play a role in oogenesis. In mice, it is essential for folliculogenesis and regulation of oocyte-specific genes. Defects in this gene result in premature ovarian failure type 5.[provided by RefSeq, May 2011]

Entrez Gene ID

135935

UniProt ID

O60393

Alternative Names

NOBOX Oogenesis Homeobox; Newborn Ovary Homeobox-Encoding Gene; Newborn Ovary Homeobox-Encoding; Homeobox Protein NOBOX; TCAG_12042; OG-2; OG2X; POF5; OG2;

Function

Transcription factor which may play a role in oogenesis. Binds preferentially to the DNA sequences 5'-TAATTG-3', 5'-TAGTTG-3' and 5'-TAATTA-3'.

Biological Process

OogenesisIEA:UniProtKB-KW
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus

Involvement in disease

Premature ovarian failure 5 (POF5):
An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

References

Jordan, P., Verebi, C., Perol, S., Grotto, S., Fouveaut, C., Christin-Maitre, S., ... & Bienvenu, T. (2024). NOBOX gene variants in premature ovarian insufficiency: ethnicity-dependent insights. Journal of Assisted Reproduction and Genetics, 41(1), 135-146.

Wu, K., Zhai, Y., Qin, M., Zhao, C., Ai, N., He, J., & Ge, W. (2023). Genetic evidence for differential functions of figla and nobox in zebrafish ovarian differentiation and folliculogenesis. Communications Biology, 6(1), 1185.

Patton, B. K., Madadi, S., Briley, S. M., Ahmed, A. A., & Pangas, S. A. (2023). Sumoylation regulates functional properties of the oocyte transcription factors SOHLH1 and NOBOX. FASEB journal: official publication of the Federation of American Societies for Experimental Biology, 37(2), e22747.

Qin, M., Xie, Q., Wu, K., Zhou, X., & Ge, W. (2022). Loss of Nobox prevents ovarian differentiation from juvenile ovaries in zebrafish. Biology of Reproduction, 106(6), 1254-1266.

Yao, C., Lu, L., Ji, Y., Zhang, Y., Li, W., Shi, Y., ... & Xia, F. (2022). Hypo-Hydroxymethylation of Nobox is Associated with Ovarian Dysfunction in Rat Offspring Exposed to Prenatal Hypoxia. Reproductive Sciences, 29(5), 1424-1436.

Sassi, A., Désir, J., Duerinckx, S., Soblet, J., Van Dooren, S., Bonduelle, M., ... & Delbaere, A. (2021). Compound heterozygous null mutations of NOBOX in sisters with delayed puberty and primary amenorrhea. Molecular genetics & genomic medicine, 9(10), e1776.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NOBOX Recombinant Antibody (A-5)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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