Mouse Anti-NOTCH2NL Recombinant Antibody (S1) (CBMAB-A6056-LY)

Name: Mouse Anti-NOTCH2NL Recombinant Antibody (S1)
SKU: CBMAB-A6056-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

WB, ELISA

Immunogen

NOTCH2NL (AAH19835, 1 a.a. ~ 236 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Notch homolog 2 (Drosophila) N-terminal like

Entrez Gene ID

388677

UniProt ID

Q7Z3S9

Alternative Names

N2N

Function

Human-specific protein that promotes neural progenitor proliferation and evolutionary expansion of the brain neocortex by regulating the Notch signaling pathway (PubMed:29856954, PubMed:29856955, PubMed:29561261).
Able to promote neural progenitor self-renewal, possibly by down-regulating neuronal differentiation genes, thereby delaying the differentiation of neuronal progenitors and leading to an overall final increase in neuronal production (PubMed:29856954).
Acts by enhancing the Notch signaling pathway via two different mechanisms that probably work in parallel to reach the same effect (PubMed:29856954).
Enhances Notch signaling pathway in a non-cell-autonomous manner via direct interaction with NOTCH2 (PubMed:29856954).
Also promotes Notch signaling pathway in a cell-autonomous manner through inhibition of cis DLL1-NOTCH2 interactions, which promotes neuronal differentiation (By similarity).

Biological Process

Cell differentiationIEA:UniProtKB-KW
Cerebral cortex developmentManual Assertion Based On ExperimentIDA:UniProtKB
Notch signaling pathwayIEA:UniProtKB-KW
Positive regulation of Notch signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Secreted
Cytoplasm

Involvement in disease

Defects in NOTCH2NLA may be a cause of chromosome 1q21.1 deletion/duplication syndrome (PubMed:29856954). Deletions of NOTCH2NL (NOTCH2NLA, NOTCH2NLB and/or NOTCH2NLC) are present in patients affected by microcephaly, whereas macrocephaly is observed in patients with NOTCH2NL duplications (PubMed:29856954).

PTM

Cleaved by ELANE.

Summan, S. K., Chow, B., Millar, R., Chew, S. K., East, P., Hynds, R., ... & Hiley, C. T. (2022). Notch2NL is a novel regulator of radiation sensitivity in non-small cell lung cancer and a positive regulator of MYC activity. Cancer Research, 82(12_Supplement), 204-204.

Funato, K., Smith, R. C., Saito, Y., & Tabar, V. (2021). Dissecting the impact of regional identity and the oncogenic role of human-specific NOTCH2NL in an hESC model of H3. 3G34R-mutant glioma. Cell Stem Cell, 28(5), 894-905.

Lodewijk, G. A., Fernandes, D. P., Vretzakis, I., Savage, J. E., & Jacobs, F. M. (2020). Evolution of human brain size-associated NOTCH2NL genes proceeds toward reduced protein levels. Molecular biology and evolution, 37(9), 2531-2548.

Fiddes, I. T., Pollen, A. A., Davis, J. M., & Sikela, J. M. (2019). Paired involvement of human-specific Olduvai domains and NOTCH2NL genes in human brain evolution. Human genetics, 138, 715-721.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-NOTCH2NL Recombinant Antibody (CBWJN-0344) (CAT#: CBMAB-N3042-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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