Human Recombinant NOTCH2NL protein, Fc Tag (V2LY-0526-LY5821)

Name: Human Recombinant NOTCH2NL protein, Fc Tag
SKU: V2LY-0526-LY5821
Rating: 5 (1 reviews)

See all NOTCH2NL antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

Fc Tag

Protein Construction

This product is Human Recombinant NOTCH2NL protein, Fc Tag consist of Amino Acid: 1-236 and predicts a molecular mass of 52.5 kDa.

Molecule Mass

52.5 kDa

Sequence

Amino Acid: 1-236

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Notch homolog 2 (Drosophila) N-terminal like

Function

Human-specific protein that promotes neural progenitor proliferation and evolutionary expansion of the brain neocortex by regulating the Notch signaling pathway (PubMed:29856954, PubMed:29856955, PubMed:29561261).
Able to promote neural progenitor self-renewal, possibly by down-regulating neuronal differentiation genes, thereby delaying the differentiation of neuronal progenitors and leading to an overall final increase in neuronal production (PubMed:29856954).
Acts by enhancing the Notch signaling pathway via two different mechanisms that probably work in parallel to reach the same effect (PubMed:29856954).
Enhances Notch signaling pathway in a non-cell-autonomous manner via direct interaction with NOTCH2 (PubMed:29856954).
Also promotes Notch signaling pathway in a cell-autonomous manner through inhibition of cis DLL1-NOTCH2 interactions, which promotes neuronal differentiation (By similarity).

Biological Process

Cell differentiationIEA:UniProtKB-KW
Cerebral cortex developmentManual Assertion Based On ExperimentIDA:UniProtKB
Notch signaling pathwayIEA:UniProtKB-KW
Positive regulation of Notch signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Secreted
Cytoplasm

Involvement in disease

Defects in NOTCH2NLA may be a cause of chromosome 1q21.1 deletion/duplication syndrome (PubMed:29856954). Deletions of NOTCH2NL (NOTCH2NLA, NOTCH2NLB and/or NOTCH2NLC) are present in patients affected by microcephaly, whereas macrocephaly is observed in patients with NOTCH2NL duplications (PubMed:29856954).

PTM

Cleaved by ELANE.

Summan, S. K., Chow, B., Millar, R., Chew, S. K., East, P., Hynds, R., ... & Hiley, C. T. (2022). Notch2NL is a novel regulator of radiation sensitivity in non-small cell lung cancer and a positive regulator of MYC activity. Cancer Research, 82(12_Supplement), 204-204.

Funato, K., Smith, R. C., Saito, Y., & Tabar, V. (2021). Dissecting the impact of regional identity and the oncogenic role of human-specific NOTCH2NL in an hESC model of H3. 3G34R-mutant glioma. Cell Stem Cell, 28(5), 894-905.

Lodewijk, G. A., Fernandes, D. P., Vretzakis, I., Savage, J. E., & Jacobs, F. M. (2020). Evolution of human brain size-associated NOTCH2NL genes proceeds toward reduced protein levels. Molecular biology and evolution, 37(9), 2531-2548.

Fiddes, I. T., Pollen, A. A., Davis, J. M., & Sikela, J. M. (2019). Paired involvement of human-specific Olduvai domains and NOTCH2NL genes in human brain evolution. Human genetics, 138, 715-721.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant NOTCH2NL protein, Fc Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-NOTCH2NL Recombinant Antibody (CBWJN-0344) (CAT#: CBMAB-N3042-WJ)

Mouse Anti-NOTCH2NL Recombinant Antibody (S1) (CAT#: CBMAB-A6056-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry