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Hamster Anti-NOTCH4 Recombinant Antibody (CBWJN-0610) (CBMAB-N0476-WJ)

This product is a Hamster antibody that recognizes NOTCH4. The antibody CBWJN-0610 can be used for immunoassay techniques such as: FC.
See all NOTCH4 antibodies

Summary

Host Animal
Hamster
Specificity
Human
Clone
CBWJN-0610
Antibody Isotype
IgG
Application
FC

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1.0 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Notch 4
Introduction
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
Entrez Gene ID
4855
UniProt ID
Q99466
Alternative Names
Notch 4; INT3; Neurogenic Locus Notch Homolog Protein 4; Notch (Drosophila) Homolog 4; Notch Homolog 4 (Drosophila); Notch Homolog 4; HNotch4;
Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).
Biological Process
Branching involved in blood vessel morphogenesisISS:UniProtKB
Cell differentiation1 PublicationNAS:UniProtKB
Cell fate determinationManual Assertion Based On ExperimentTAS:UniProtKB
Endothelial cell morphogenesisIEA:Ensembl
Epithelial to mesenchymal transitionManual Assertion Based On ExperimentIMP:ARUK-UCL
HemopoiesisManual Assertion Based On ExperimentTAS:UniProtKB
Mammary gland developmentManual Assertion Based On ExperimentIDA:UniProtKB
Morphogenesis of a branching structureISS:UniProtKB
Negative regulation of cell adhesion molecule productionManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of cell differentiation1 PublicationNAS:UniProtKB
Negative regulation of cell-cell adhesion mediated by cadherinManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of endothelial cell differentiationISS:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of transcription of Notch receptor targetManual Assertion Based On ExperimentTAS:BHF-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentTAS:UniProtKB
Vasculature developmentISS:UniProtKB
Wound healingIEA:Ensembl
Cellular Location
Cell membrane
Notch 4 intracellular domain
Nucleus
Following proteolytical processing NICD is translocated to the nucleus.
Topology
Extracellular: 24-1447
Helical: 1448-1468
Cytoplasmic: 1469-2003
PTM
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).
Phosphorylated.

Guo, M., Zhang, M., Cao, X., Fang, X., Li, K., Qin, L., ... & Li, X. (2022). Notch4 mediates vascular remodeling via ERK/JNK/P38 MAPK signaling pathways in hypoxic pulmonary hypertension. Respiratory Research, 23(1), 1-18.

Muley, A., Kim Uh, M., Salazar-De Simone, G., Swaminathan, B., James, J. M., Murtomaki, A., ... & Shawber, C. J. (2022). Unique functions for Notch4 in murine embryonic lymphangiogenesis. Angiogenesis, 1-20.

Scheurlen, K. M., Chariker, J. H., Kanaan, Z., Littlefield, A. B., George, J. B., Seraphine, C., ... & Galandiuk, S. (2022). The NOTCH4-GATA4-IRG1 axis as a novel target in early-onset colorectal cancer. Cytokine & Growth Factor Reviews.

Xiu, M., Zeng, X., Shan, R., Wen, W., Li, J., & Wan, R. (2021). Targeting Notch4 in cancer: molecular mechanisms and therapeutic perspectives. Cancer Management and Research, 7033-7045.

Long, J., Wang, D., Yang, X., Wang, A., Lin, Y., Zheng, M., ... & Zhao, H. (2021). Identification of NOTCH4 mutation as a response biomarker for immune checkpoint inhibitor therapy. BMC medicine, 19(1), 1-14.

Harb, H., Benamar, M., Lai, P. S., Contini, P., Griffith, J. W., Crestani, E., ... & Chatila, T. A. (2021). Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections. Immunity, 54(6), 1186-1199.

López-López, S., Romero de Ávila, M. J., Hernández de León, N. C., Ruiz-Marcos, F., Baladrón, V., Nueda, M. L., ... & Díaz-Guerra, M. J. M. (2021). NOTCH4 exhibits anti-inflammatory activity in activated macrophages by interfering with interferon-γ and TLR4 signaling. Frontiers in Immunology, 12, 734966.

Harb, H., Stephen-Victor, E., Crestani, E., Benamar, M., Massoud, A., Cui, Y., ... & Chatila, T. A. (2020). A regulatory T cell Notch4–GDF15 axis licenses tissue inflammation in asthma. Nature immunology, 21(11), 1359-1370.

Zhou, L., Wang, D., Sheng, D., Xu, J., Chen, W., Qin, Y., ... & Zhang, L. (2020). NOTCH4 maintains quiescent mesenchymal-like breast cancer stem cells via transcriptionally activating SLUG and GAS1 in triple-negative breast cancer. Theranostics, 10(5), 2405.

Lv, H., Nan, Z., Jiang, P., Wang, Z., Song, M., Ding, H., ... & Hu, Y. (2019). Vascular endothelial growth factor 165 inhibits pro-fibrotic differentiation of stromal cells via the DLL4/Notch4/smad7 pathway. Cell Death & Disease, 10(9), 681.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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