Human Recombinant NOTCH4 protein, His Tag (V2LY-0526-LY5822)

Name: Human Recombinant NOTCH4 protein, His Tag
SKU: V2LY-0526-LY5822
Rating: 5 (1 reviews)

See all NOTCH4 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant NOTCH4 protein, His Tag consist of Amino Acid: 1-637 and predicts a molecular mass of 66.1 kDa.

Molecule Mass

66.1 kDa

Sequence

Amino Acid: 1-637

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Notch 4

Function

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).

Biological Process

Branching involved in blood vessel morphogenesisISS:UniProtKB
Cell differentiation1 PublicationNAS:UniProtKB
Cell fate determinationManual Assertion Based On ExperimentTAS:UniProtKB
Endothelial cell morphogenesisIEA:Ensembl
Epithelial to mesenchymal transitionManual Assertion Based On ExperimentIMP:ARUK-UCL
HemopoiesisManual Assertion Based On ExperimentTAS:UniProtKB
Mammary gland developmentManual Assertion Based On ExperimentIDA:UniProtKB
Morphogenesis of a branching structureISS:UniProtKB
Negative regulation of cell adhesion molecule productionManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of cell differentiation1 PublicationNAS:UniProtKB
Negative regulation of cell-cell adhesion mediated by cadherinManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of endothelial cell differentiationISS:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of transcription of Notch receptor targetManual Assertion Based On ExperimentTAS:BHF-UCL
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentTAS:UniProtKB
Vasculature developmentISS:UniProtKB
Wound healingIEA:Ensembl

Cellular Location

Cell membrane
Notch 4 intracellular domain
Nucleus
Following proteolytical processing NICD is translocated to the nucleus.

Topology

Extracellular: 24-1447
Helical: 1448-1468
Cytoplasmic: 1469-2003

PTM

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).
Phosphorylated.

Guo, M., Zhang, M., Cao, X., Fang, X., Li, K., Qin, L., ... & Li, X. (2022). Notch4 mediates vascular remodeling via ERK/JNK/P38 MAPK signaling pathways in hypoxic pulmonary hypertension. Respiratory Research, 23(1), 1-18.

Muley, A., Kim Uh, M., Salazar-De Simone, G., Swaminathan, B., James, J. M., Murtomaki, A., ... & Shawber, C. J. (2022). Unique functions for Notch4 in murine embryonic lymphangiogenesis. Angiogenesis, 1-20.

Scheurlen, K. M., Chariker, J. H., Kanaan, Z., Littlefield, A. B., George, J. B., Seraphine, C., ... & Galandiuk, S. (2022). The NOTCH4-GATA4-IRG1 axis as a novel target in early-onset colorectal cancer. Cytokine & Growth Factor Reviews.

Xiu, M., Zeng, X., Shan, R., Wen, W., Li, J., & Wan, R. (2021). Targeting Notch4 in cancer: molecular mechanisms and therapeutic perspectives. Cancer Management and Research, 7033-7045.

Long, J., Wang, D., Yang, X., Wang, A., Lin, Y., Zheng, M., ... & Zhao, H. (2021). Identification of NOTCH4 mutation as a response biomarker for immune checkpoint inhibitor therapy. BMC medicine, 19(1), 1-14.

Harb, H., Benamar, M., Lai, P. S., Contini, P., Griffith, J. W., Crestani, E., ... & Chatila, T. A. (2021). Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections. Immunity, 54(6), 1186-1199.

López-López, S., Romero de Ávila, M. J., Hernández de León, N. C., Ruiz-Marcos, F., Baladrón, V., Nueda, M. L., ... & Díaz-Guerra, M. J. M. (2021). NOTCH4 exhibits anti-inflammatory activity in activated macrophages by interfering with interferon-γ and TLR4 signaling. Frontiers in Immunology, 12, 734966.

Harb, H., Stephen-Victor, E., Crestani, E., Benamar, M., Massoud, A., Cui, Y., ... & Chatila, T. A. (2020). A regulatory T cell Notch4–GDF15 axis licenses tissue inflammation in asthma. Nature immunology, 21(11), 1359-1370.

Zhou, L., Wang, D., Sheng, D., Xu, J., Chen, W., Qin, Y., ... & Zhang, L. (2020). NOTCH4 maintains quiescent mesenchymal-like breast cancer stem cells via transcriptionally activating SLUG and GAS1 in triple-negative breast cancer. Theranostics, 10(5), 2405.

Lv, H., Nan, Z., Jiang, P., Wang, Z., Song, M., Ding, H., ... & Hu, Y. (2019). Vascular endothelial growth factor 165 inhibits pro-fibrotic differentiation of stromal cells via the DLL4/Notch4/smad7 pathway. Cell Death & Disease, 10(9), 681.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant NOTCH4 protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-NOTCH4 Recombinant Antibody (CBWJN-0659) (CAT#: CBMAB-N0477-WJ)

Hamster Anti-NOTCH4 Recombinant Antibody (CBWJN-0610) (CAT#: CBMAB-N0476-WJ)

Mouse Anti-NOTCH4 Recombinant Antibody (CBT3673) (CAT#: V2LY-0625-LY1975)

Hamster Anti-NOTCH4 Recombinant Antibody (CBWJN-0104) (CAT#: CBMAB-N3055-WJ)

Rat Anti-NOTCH4 Recombinant Antibody (7H240) (CAT#: CBMAB-N3056-WJ)

Mouse Anti-NOTCH4 Recombinant Antibody (CBT2054) (CAT#: V2LY-0625-LY1994)

Rat Anti-NOTCH4 Recombinant Antibody (CBWJN-1482) (CAT#: CBMAB-N3053-WJ)

Mouse Anti-NOTCH4 Recombinant Antibody (7H44) (CAT#: CBMAB-N3057-WJ)

Armenian Hamster Anti-NOTCH4 Recombinant Antibody (HMN4-14) (CAT#: CBMAB-1074-LY)

Mouse Anti-NOTCH4 Recombinant Antibody (L5C5) (CAT#: CBMAB-N3060-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry