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Rabbit Anti-NOVA1 Recombinant Antibody (BA0136) (CBMAB-0531CQ)

This product is a rabbit antibody that recognizes NOVA1. The antibody BA0136 can be used for immunoassay techniques such as: IHC-P, WB.
See all NOVA1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
BA0136
Antibody Isotype
IgG
Application
IHC-P, WB

Basic Information

Immunogen
Human Syntaxin aa 450 to the C-terminus
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NOVA Alternative Splicing Regulator 1
Introduction
This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. Using antisera from patients with a paraneoplastic neurologic disorder involving the subcortical motor system. The NOVA1 gene encodes a neuron-specific RNA-binding protein that is inhibited by paraneoplastic antibodies. Fletcher et al. (1997) showed that the mouse homolog maps to mouse chromosome 12 and suggested that the human gene is probably located on 14q.These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.
Entrez Gene ID
Human4857
Mouse664883
Rat298992
UniProt ID
HumanP51513
MouseQ9JKN6
RatQ80WA4
Alternative Names
Nova-1
Function
Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons (PubMed:10811881).
Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons. Binding to an exonic 5'-YCAY-3' cluster changed the protein complexes assembled on pre-mRNA, blocking U1 snRNP (small nuclear ribonucleoprotein) binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhanced spliceosome assembly and exon inclusion. With NOVA1, they perform unique biological functions in different brain areas and cell types. Autoregulates its own expression by acting as a splicing repressor. Acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer. Acts to regulate a novel glycine receptor alpha-2 chain splice variant (alpha-2N) in developing spinal cord (By similarity).
Biological Process
mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Nervous system developmentIEA:UniProtKB-KW
Regulation of alternative mRNA splicing, via spliceosomeISS:UniProtKB
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Regulation of RNA metabolic processManual Assertion Based On ExperimentIBA:GO_Central
RNA processingManual Assertion Based On ExperimentTAS:ProtInc
RNA splicingManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Nucleus

Pedraza-Arevalo, S., Alors-Pérez, E., Blázquez-Encinas, R., Herrera-Martínez, A. D., Jiménez-Vacas, J. M., Fuentes-Fayos, A. C., ... & Castaño, J. P. (2023). Spliceosomic dysregulation unveils NOVA1 as a candidate actionable therapeutic target in pancreatic neuroendocrine tumors. Translational Research, 251, 63-73.

Tajima, Y., Ito, K., Yuan, Y., Frank, M. O., Saito, Y., & Darnell, R. B. (2023). NOVA1 acts on Impact to regulate hypothalamic function and translation in inhibitory neurons. Cell Reports, 42(2).

Krach, F., Wheeler, E. C., Regensburger, M., Boerstler, T., Wend, H., Vu, A. Q., ... & Winner, B. (2022). Aberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis. Acta Neuropathologica, 144(3), 413-435.

Brahma, M. K., Xiao, P., Popa, M., Negueruela, J., Vandenbempt, V., Demine, S., ... & Gurzov, E. N. (2022). Nova1 or Bim Deficiency in Pancreatic β-Cells Does Not Alter Multiple Low-Dose Streptozotocin-Induced Diabetes and Diet-Induced Obesity in Mice. Nutrients, 14(18), 3866.

Qu, L., Tian, Y., Wang, F., & Li, Z. (2022). NOVA1 promotes NSCLC proliferation and invasion by activating Wnt/β-catenin signaling. BMC cancer, 22(1), 1-13.

Trujillo, C. A., Rice, E. S., Schaefer, N. K., Chaim, I. A., Wheeler, E. C., Madrigal, A. A., ... & Muotri, A. R. (2021). Reintroduction of the archaic variant of NOVA1 in cortical organoids alters neurodevelopment. Science, 371(6530), eaax2537.

Maricic, T., Helmbrecht, N., Riesenberg, S., Macak, D., Kanis, P., Lackner, M., ... & Pääbo, S. (2021). Comment on “Reintroduction of the archaic variant of NOVA1 in cortical organoids alters neurodevelopment”. Science, 374(6565), eabi6060.

Zhang, T., Chen, S., Peng, Y., Wang, C., Cheng, X., Zhao, R., & Liu, K. (2021). NOVA1-mediated SORBS2 isoform promotes colorectal cancer migration by activating the Notch pathway. Frontiers in Cell and Developmental Biology, 9, 673873.

Jin, L., Li, H., Wang, J., Lin, D., Yin, K., Lin, L., ... & Teng, L. (2019). MicroRNA‐193a‐5p exerts a tumor suppressor role in glioblastoma via modulating NOVA1. Journal of cellular biochemistry, 120(4), 6188-6197.

Sayed, M. E., Yuan, L., Robin, J. D., Tedone, E., Batten, K., Dahlson, N., ... & Ludlow, A. T. (2019). NOVA1 directs PTBP1 to hTERT pre-mRNA and promotes telomerase activity in cancer cells. Oncogene, 38(16), 2937-2952.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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