Mouse Anti-NRARP Recombinant Antibody (C-12) (CBMAB-N3516-WJ)

Name: Mouse Anti-NRARP Recombinant Antibody (C-12)
SKU: CBMAB-N3516-WJ
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse, Rat, Human

Clone

C-12

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Mouse, Rat, Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

NOTCH Regulated Ankyrin Repeat Protein

Introduction

Downstream effector of Notch signaling. Involved in the regulation of liver cancer cells self-renewal (PubMed:25985737). Involved in angiogenesis acting downstream of Notch at branch points to regulate vascular density. Proposed to integrate endothelial Notch and Wnt signaling to control stalk cell proliferation and to stablilize new endothelial connections during angiogenesis (PubMed:19154719). During somitogenesis involved in maintenance of proper somite segmentation and proper numbers of somites and vertebrae. Required for proper anterior-posterior somite patterning. Proposed to function in a negative feedback loop to destabilize Notch 1 intracellular domain (NICD) and downregulate the Notch signal, preventing expansion of the Notch signal into the anterior somite domain (By similarity).

Entrez Gene ID

Human	441478
Mouse	67122
Rat	499745

UniProt ID

Human	Q7Z6K4
Mouse	Q91ZA8
Rat	D3Z9S9

Alternative Names

NOTCH Regulated Ankyrin Repeat Protein;

Function

Downstream effector of Notch signaling. Involved in the regulation of liver cancer cells self-renewal (PubMed:25985737).
Involved in angiogenesis acting downstream of Notch at branch points to regulate vascular density. Proposed to integrate endothelial Notch and Wnt signaling to control stalk cell proliferation and to stablilize new endothelial connections during angiogenesis (PubMed:19154719).
During somitogenesis involved in maintenance of proper somite segmentation and proper numbers of somites and vertebrae. Required for proper anterior-posterior somite patterning. Proposed to function in a negative feedback loop to destabilize Notch 1 intracellular domain (NICD) and down-regulate the Notch signal, preventing expansion of the Notch signal into the anterior somite domain (By similarity).

Biological Process

Blood vessel endothelial cell proliferation involved in sprouting angiogenesisManual Assertion Based On ExperimentIBA:GO_Central
Branching involved in blood vessel morphogenesisManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of Notch signaling pathwayManual Assertion Based On ExperimentIMP:MGI
Negative regulation of Notch signaling pathway involved in somitogenesisIEA:Ensembl
Negative regulation of T cell differentiationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Notch signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIMP:MGI
Positive regulation of endothelial cell proliferationManual Assertion Based On ExperimentIBA:GO_Central
Somite rostral/caudal axis specificationIEA:Ensembl

More Infomation

References

Poodineh, J., Sirati‐Sabet, M., Rajabibazl, M., Ghasemian, M., & Mohammadi‐Yeganeh, S. (2022). Downregulation of NRARP exerts anti‐tumor activities in the breast tumor cells depending on Wnt/β‐catenin‐mediated signals: The role of miR‐130a‐3p. Chemical Biology & Drug Design, 100(3), 334-345.

Rajabibazl, M., Poodineh, J., Sirati-Sabet, M., & Mohammadi-Yeganeh, S. (2022). Downregulation of NRARP exerts anti-tumor activities in the breast tumor cells depending on Wnt/ꞵ-catenin mediated signals; the role of miR-130a-3p.

Pinto, I., Duque, M., Gonçalves, J., Akkapeddi, P., Oliveira, M. L., Cabrita, R., ... & Fragoso, R. (2020). NRARP displays either pro-or anti-tumoral roles in T-cell acute lymphoblastic leukemia depending on Notch and Wnt signaling. Oncogene, 39(5), 975-986.

John, A. A., Prakash, R., & Singh, D. (2019). miR-487b-3p impairs osteoblastogenesis by targeting Notch-regulated ankyrin-repeat protein (Nrarp). Journal of Endocrinology, 241(3), 249-263.

Jarrett, S. M., Seegar, T. C., Andrews, M., Adelmant, G., Marto, J. A., Aster, J. C., & Blacklow, S. C. (2019). Extension of the Notch intracellular domain ankyrin repeat stack by NRARP promotes feedback inhibition of Notch signaling. Science signaling, 12(606), eaay2369.

Jarrett, S. M., Seegar, T. C., Andrews, M., Adelmant, G., Marto, J. A., Aster, J. C., & Blacklow, S. C. (2019). Molecular basis for feedback inhibition of Notch signaling by the Notch regulated ankyrin repeat protein NRARP. bioRxiv, 645937.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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