Recombinant Mouse Anti-NSL1 Recombinant Antibody (QL24-1) (CBMAB-XB0888-YC)

Provided herein is a Mouse Recombinant Antibody against NSL1, MIS12 Kinetochore Complex Component. The antibody can be used for immunoassay techniques, such as IF, IP, WB.
See all NSL1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

QL24-1

Antibody Isotype

IgG1, κ

Application

IF, IP, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

NSL1, MIS12 Kinetochore Complex Component

Introduction

NSL1 is two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. There is a pseudogene of the 3' UTR region of this gene on chromosome X.

Entrez Gene ID

25936

UniProt ID

Q96IY1

Alternative Names

NSL1, MIS12 Kinetochore Complex Component; C1orf48; NSL1, MIND Kinetochore Complex Component, Homolog (S. Cerevisiae); NSL1, MIND Kinetochore Complex Component, Homolog; Kinetochore-Associated Protein NSL1 Homolog; Chromosome 1 Open Reading Frame 48; MIS14; DC8;

Function

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.

Biological Process

Cell divisionIEA:UniProtKB-KW
Mitotic sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Chromosome, centromere, kinetochore
Associated with the kinetochore (PubMed:15502821, PubMed:16585270).

References

Yatskevich, S., Yang, J., Bellini, D., Zhang, Z., & Barford, D. (2024). Structure of the human outer kinetochore KMN network complex. Nature Structural & Molecular Biology, 1-10.

Sridhar, S., & Fukagawa, T. (2022). Kinetochore architecture employs diverse linker strategies across evolution. Frontiers in Cell and Developmental Biology, 10, 862637.

Bolanos-Garcia, V. M. (2022). On the Regulation of Mitosis by the Kinetochore, a Macromolecular Complex and Organising Hub of Eukaryotic Organisms. In Macromolecular Protein Complexes IV: Structure and Function (pp. 235-267). Cham: Springer International Publishing.

Allipra, S., Anirudhan, K., Shivanandan, S., Raghunathan, A., & Maruthachalam, R. (2022). The kinetochore protein NNF1 has a moonlighting role in the vegetative development of Arabidopsis thaliana. The Plant Journal, 109(5), 1064-1085.

Guo, Y. (2019). The Architectural Changes of Mis12 Complex under Different Tension Levels (Doctoral dissertation, Johns Hopkins University).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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