Rabbit Anti-NTHL1 Recombinant Antibody (
22H10) (V2LY-0725-LY1399)

Name: Rabbit Anti-NTHL1 Recombinant Antibody (22H10)
SKU: V2LY-0725-LY1399
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-NTHL1 Recombinant Antibody (22H10) (V2LY-0725-LY1399) in WB

Western Blot
Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate
All lanes: NTHL1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35, 34, 33 kDa
Observed band size: 35 kDa

Rabbit Anti-NTHL1 Recombinant Antibody (22H10) (V2LY-0725-LY1399) in IHC

IHC image of V2LY-0725-LY1399 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-NTHL1 Recombinant Antibody (22H10) (V2LY-0725-LY1399) in IF

Immunofluorescence staining of PC-3 cell with V2LY-0725-LY1399 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 604-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-NTHL1 Recombinant Antibody (22H10) (V2LY-0725-LY1399) in FC

Overlay Peak curve showing HepG2 cells stained with V2LY-0725-LY1399 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

22H10

Application

ELISA, WB, IHC, IF, FC

Immunogen

A synthesized peptide derived from human NTHL1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Nth Like Dna Glycosylase 1

Entrez Gene ID

4913

UniProt ID

P78549

Function

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage (PubMed:9927729).
The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.

Biological Process

Base-excision repair, AP site formationManual Assertion Based On ExperimentIDA:UniProtKB
DepyrimidinationTAS:Reactome
Nucleotide-excision repair, DNA incision, 5'-to lesionManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus
Mitochondrion

Involvement in disease

Familial adenomatous polyposis 3 (FAP3):
A form of familial adenomatous polyposis, a condition characterized by the development of multiple colorectal adenomatous polyps, benign neoplasms derived from glandular epithelium. Some affected individuals may develop colorectal carcinoma.

Salo-Mullen, E. E., Maio, A., Mukherjee, S., Bandlamudi, C., Shia, J., Kemel, Y., ... & Stadler, Z. K. (2021). Prevalence and characterization of biallelic and monoallelic NTHL1 and MSH3 variant carriers from a pan-cancer patient population. JCO precision oncology, 5, 455-465.

Magrin, L., Fanale, D., Brando, C., Fiorino, A., Corsini, L. R., Sciacchitano, R., ... & Bazan, V. (2021). POLE, POLD1, and NTHL1: The last but not the least hereditary cancer-predisposing genes. Oncogene, 40(40), 5893-5901.

Carroll, B. L., Zahn, K. E., Hanley, J. P., Wallace, S. S., Dragon, J. A., & Doublié, S. (2021). Caught in motion: human NTHL1 undergoes interdomain rearrangement necessary for catalysis. Nucleic Acids Research, 49(22), 13165-13178.

Das, L., Quintana, V. G., & Sweasy, J. B. (2020). NTHL1 in genomic integrity, aging and cancer. DNA repair, 93, 102920.

Kuiper, R. P., Nielsen, M., De Voer, R. M., & Hoogerbrugge, N. (2020). NTHL1 tumor syndrome.

Grolleman, J. E., De Voer, R. M., Elsayed, F. A., Nielsen, M., Weren, R. D., Palles, C., ... & Kuiper, R. P. (2019). Mutational signature analysis reveals NTHL1 deficiency to cause a multi-tumor phenotype. Cancer cell, 35(2), 256-266.

Belhadj, S., Quintana, I., Mur, P., Munoz-Torres, P. M., Alonso, M. H., Navarro, M., ... & Valle, L. (2019). NTHL1 biallelic mutations seldom cause colorectal cancer, serrated polyposis or a multi-tumor phenotype, in absence of colorectal adenomas. Scientific Reports, 9(1), 9020.

Terradas, M., Munoz‐Torres, P. M., Belhadj, S., Aiza, G., Navarro, M., Brunet, J., ... & Valle, L. (2019). Contribution to colonic polyposis of recently proposed predisposing genes and assessment of the prevalence of NTHL1‐and MSH3‐associated polyposes. Human Mutation, 40(11), 1910-1923.

Groves, A., Gleeson, M., & Spigelman, A. D. (2019). NTHL1-associate polyposis: first Australian case report. Familial cancer, 18(2), 179-182.

Altaraihi, M., Gerdes, A. M., & Wadt, K. (2019). A new family with a homozygous nonsense variant in NTHL1 further delineated the clinical phenotype of NTHL1-associated polyposis. Human Genome Variation, 6(1), 46.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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