Mouse Anti-NTHL1 Recombinant Antibody (CBWJN-0495) (CBMAB-N3742-WJ)

Name: Mouse Anti-NTHL1 Recombinant Antibody (CBWJN-0495)
SKU: CBMAB-N3742-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-0495

Application

Dot, WB

Specificity

Human, Mouse, Rat

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% sodium azide

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nth Like Dna Glycosylase 1

Introduction

The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008]

Entrez Gene ID

Human	4913
Mouse	18207
Rat	29541

UniProt ID

Human	P78549
Mouse	O35980
Rat	D4A4E8

Alternative Names

Nth Like DNA Glycosylase 1; Bifunctional DNA N-Glycosylase/DNA-(Apurinic Or Apyrimidinic Site) Lyase; DNA Glycosylase/AP Lyase; OCTS3; HNTH1; NTH1; Bifunctional DNA N-Glycoslyase/DNA-(Apurinic Or Apyrimidinic Site) Lyase; Nth Endonuclease III-Like 1 (E. Coli);

Function

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage (PubMed:9927729).
The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.

Biological Process

Base-excision repair, AP site formationManual Assertion Based On ExperimentIDA:UniProtKB
DepyrimidinationTAS:Reactome
Nucleotide-excision repair, DNA incision, 5'-to lesionManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus
Mitochondrion

Involvement in disease

Familial adenomatous polyposis 3 (FAP3):
A form of familial adenomatous polyposis, a condition characterized by the development of multiple colorectal adenomatous polyps, benign neoplasms derived from glandular epithelium. Some affected individuals may develop colorectal carcinoma.

Salo-Mullen, E. E., Maio, A., Mukherjee, S., Bandlamudi, C., Shia, J., Kemel, Y., ... & Stadler, Z. K. (2021). Prevalence and characterization of biallelic and monoallelic NTHL1 and MSH3 variant carriers from a pan-cancer patient population. JCO precision oncology, 5, 455-465.

Magrin, L., Fanale, D., Brando, C., Fiorino, A., Corsini, L. R., Sciacchitano, R., ... & Bazan, V. (2021). POLE, POLD1, and NTHL1: The last but not the least hereditary cancer-predisposing genes. Oncogene, 40(40), 5893-5901.

Carroll, B. L., Zahn, K. E., Hanley, J. P., Wallace, S. S., Dragon, J. A., & Doublié, S. (2021). Caught in motion: human NTHL1 undergoes interdomain rearrangement necessary for catalysis. Nucleic Acids Research, 49(22), 13165-13178.

Das, L., Quintana, V. G., & Sweasy, J. B. (2020). NTHL1 in genomic integrity, aging and cancer. DNA repair, 93, 102920.

Kuiper, R. P., Nielsen, M., De Voer, R. M., & Hoogerbrugge, N. (2020). NTHL1 tumor syndrome.

Grolleman, J. E., De Voer, R. M., Elsayed, F. A., Nielsen, M., Weren, R. D., Palles, C., ... & Kuiper, R. P. (2019). Mutational signature analysis reveals NTHL1 deficiency to cause a multi-tumor phenotype. Cancer cell, 35(2), 256-266.

Belhadj, S., Quintana, I., Mur, P., Munoz-Torres, P. M., Alonso, M. H., Navarro, M., ... & Valle, L. (2019). NTHL1 biallelic mutations seldom cause colorectal cancer, serrated polyposis or a multi-tumor phenotype, in absence of colorectal adenomas. Scientific Reports, 9(1), 9020.

Terradas, M., Munoz‐Torres, P. M., Belhadj, S., Aiza, G., Navarro, M., Brunet, J., ... & Valle, L. (2019). Contribution to colonic polyposis of recently proposed predisposing genes and assessment of the prevalence of NTHL1‐and MSH3‐associated polyposes. Human Mutation, 40(11), 1910-1923.

Groves, A., Gleeson, M., & Spigelman, A. D. (2019). NTHL1-associate polyposis: first Australian case report. Familial cancer, 18(2), 179-182.

Altaraihi, M., Gerdes, A. M., & Wadt, K. (2019). A new family with a homozygous nonsense variant in NTHL1 further delineated the clinical phenotype of NTHL1-associated polyposis. Human Genome Variation, 6(1), 46.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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