Mouse Anti-NUDT3 Recombinant Antibody (3C5) (CBMAB-N3906-WJ)

This product is a Mouse antibody that recognizes NUDT3. The antibody 3C5 can be used for immunoassay techniques such as: ELISA, WB.
See all NUDT3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3C5

Antibody Isotype

IgG1, κ

Application

ELISA, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Concentration

0.21.0 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

nudix (nucleoside diphosphate linked moiety X)-type motif 3

Introduction

NUDT3 belongs to the MutT, or Nudix, protein family. Nudix proteins act as homeostatic checkpoints at important stages in nucleoside phosphate metabolic pathways, guarding against elevated levels of potentially dangerous intermediates, like 8-oxo-dGTP, which promotes AT-to-CG transversions (Safrany et al., 1998 [PubMed 9822604]).[supplied by OMIM, Feb 2011]

Entrez Gene ID

11165

UniProt ID

O95989

Alternative Names

Nudix Hydrolase 3; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 3; Diadenosine 5,5-P1,P6-Hexaphosphate Hydrolase 1; Nucleoside Diphosphate-Linked Moiety X Motif 3; Nudix Motif 3; DIPP-1;

Function

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction (PubMed:10419486, PubMed:12370170, PubMed:9822604, PubMed:10585413).
InsP6 (inositol hexakisphosphate) is not a substrate (PubMed:9822604).
Acts as a negative regulator of the ERK1/2 pathway (By similarity).
Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A) and diadenosine 5',5'''- P1,P5-pentaphosphate (Ap5A) being the preferred substrates (PubMed:12370170).
The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A (PubMed:12370170).
Also able to hydrolyze 5-phosphoribose 1-diphosphate (PubMed:12370170).
Acts as a decapping enzyme that modulates the stability of a subset of mRNAs implicated in cell motility (PubMed:26932476).

Biological Process

Adenosine 5'-(hexahydrogen pentaphosphate) catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Cell-cell signalingManual Assertion Based On ExperimentTAS:ProtInc
Diadenosine hexaphosphate catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Diadenosine pentaphosphate catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Diadenosine polyphosphate catabolic processManual Assertion Based On ExperimentTAS:ProtInc
Diphosphoinositol polyphosphate catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Diphosphoinositol polyphosphate metabolic processManual Assertion Based On ExperimentIBA:GO_Central
RNA decappingManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm

References

Dundr, P., Machado-Lopez, A., Mas, A., Věcková, Z., Mára, M., Richtárová, A., ... & Hojný, J. (2023). Uterine leiomyoma with RAD51B:: NUDT3 fusion: a report of 2 cases. Virchows Archiv, 1-8.

Samper-Martín, B., Sarrias, A., Lázaro, B., Pérez-Montero, M., Rodríguez-Rodríguez, R., Ribeiro, M. P., ... & Bru, S. (2021). Polyphosphate degradation by Nudt3-Zn2+ mediates oxidative stress response. Cell Reports, 37(7).

Singh, A. N., & Gasman, B. (2020). Disentangling the Genetics of Sarcopenia: prioritization of NUDT3 and KLF5 as genes for lean mass and HLA-DQB1-AS1 for hand grip strength based on associated SNPs.

Sahu, S., Wang, Z., Jiao, X., Gu, C., Jork, N., Wittwer, C., ... & Shears, S. B. (2020). InsP7 is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics. Proceedings of the National Academy of Sciences, 117(32), 19245-19253.

Hao, Q., Wang, P., Dutta, P., Chung, S., Li, Q., Wang, K., ... & Wu, Y. (2020). Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA. Cell death & disease, 11(12), 1052.

Singh, A. N., & Gasman, B. (2020). Disentangling the genetics of sarcopenia: prioritization of NUDT3 and KLF5 as genes for lean mass & HLA-DQB1-AS1 for hand grip strength with the associated enhancing SNPs & a scoring system. BMC medical genetics, 21, 1-11.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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