Mouse Anti-NUMB Recombinant Antibody (5F6E1) (CBMAB-N3972-WJ)

Name: Mouse Anti-NUMB Recombinant Antibody (5F6E1)
SKU: CBMAB-N3972-WJ
Rating: 5 (1 reviews)

See all NUMB antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5F6E1

Application

ELISA, WB, IHC

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NUMB, Endocytic Adaptor Protein

Introduction

The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Entrez Gene ID

8650

UniProt ID

P49757

Alternative Names

NUMB, Endocytic Adaptor Protein; C14orf41; H-Numb; Chromosome 14 Open Reading Frame 41; Numb (Drosophila) Homolog; Numb Homolog (Drosophila);

Function

Regulates clathrin-mediated receptor endocytosis (PubMed:18657069).
Plays a role in the process of neurogenesis (By similarity).
Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate (By similarity).
Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity (By similarity).
May also mediate local repair of brain ventricular wall damage (By similarity).

Biological Process

Adherens junction organizationIEA:Ensembl
AxonogenesisIEA:Ensembl
Lateral ventricle developmentISS:UniProtKB
Negative regulation of protein localization to plasma membraneManual Assertion Based On ExperimentIMP:UniProtKB
Neuroblast division in subventricular zoneISS:UniProtKB
Positive regulation of cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of neurogenesisISS:UniProtKB
Regulation of postsynaptic neurotransmitter receptor internalizationIEA:Ensembl

Cellular Location

Cell membrane
Endosome membrane
Localizes to perinuclear endosomes in an AAK1-dependent manner.

PTM

Phosphorylated on Ser-276 and Ser-295 by CaMK1.
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity).
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.

Ortega-Campos, S. M., & García-Heredia, J. M. (2023). The multitasker protein: a look at the multiple capabilities of NUMB. Cells, 12(2), 333.

Huang, C., Ji, C., & Wang, J. (2023). Current thoughts on cellular functions of numb-associated kinases. Molecular Biology Reports, 50(5), 4645-4652.

Xin, Y., Shang, X., Sun, X., Xu, G., Liu, Y., & Liu, Y. (2022). SLC8A1 antisense RNA 1 suppresses papillary thyroid cancer malignant progression via the FUS RNA binding protein (FUS)/NUMB like endocytic adaptor protein (Numbl) axis. Bioengineered, 13(5), 12572-12582.

Choi, H. Y., Seok, J., Kang, G. H., Lim, K. M., & Cho, S. G. (2021). The role of NUMB/NUMB isoforms in cancer stem cells. BMB reports, 54(7), 335.

Azarnia Tehran, D., Lopez-Hernandez, T., & Maritzen, T. (2019). Endocytic adaptor proteins in health and disease: Lessons from model organisms and human mutations. Cells, 8(11), 1345.

Wei, R., Liu, X., Voss, C., Qin, W., Dagnino, L., Li, L., ... & Li, S. S. C. (2019). NUMB regulates the endocytosis and activity of the anaplastic lymphoma kinase in an isoform-specific manner. Journal of Molecular Cell Biology, 11(11), 994-1005.

Vinette, M., Bélanger, M. C., Jolicoeur, C., Gabraie, M., Lachapelle, P., & Cayouette, M. (2019). AB046. The Endocytic Adaptor Protein Numb Functions in Müller Glia to Maintain Retinal Polarity and Photoreceptor Survival through the Polarity Determinant Crumbs. Annals of Eye Science, 4, AB046-AB046.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-NUMB Recombinant Antibody (5F6E1)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

NUMB Matched Antibody Pair (825) (CAT#: APMAB-825LY)

NUMB Matched Antibody Pair (826) (CAT#: APMAB-826LY)

Related Products

Mouse Anti-NUMB Recombinant Antibody (1A12) (CAT#: CBMAB-N3954-WJ)

Mouse Anti-NUMB Recombinant Antibody (1E3) (CAT#: CBMAB-N3948-WJ)

Rabbit Anti-NUMB Recombinant Antibody (CBWJN-0488) (CAT#: CBMAB-N3974-WJ)

Mouse Anti-NUMB Recombinant Antibody (4A7-A6) (CAT#: CBMAB-A6184-LY)

Mouse Anti-NUMB Recombinant Antibody (3H8) (CAT#: CBMAB-N3963-WJ)

Mouse Anti-NUMB Recombinant Antibody (CF318) (CAT#: CBMAB-FT176LY)

Rabbit Anti-NUMB Recombinant Antibody (S.925.4) (CAT#: CBMAB-N3979-WJ)

Mouse Anti-NUMB Recombinant Antibody (4C6) (CAT#: CBMAB-N3965-WJ)

NUMB Matched Antibody Pair (825) (CAT#: APMAB-825LY)

Rabbit Anti-NUMB Recombinant Antibody (C29G11) (CAT#: CBMAB-N3975-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry