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Mouse Anti-NUP50 Recombinant Antibody (G-4) (CBMAB-N4010-WJ)

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Basic Information

Host Animal
Mouse
Clone
G-4
Application
WB, IP, IF, ELISA
Specificity
Mouse, Rat, Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
nucleoporin 50kDa
Introduction
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human10762
Mouse18141
Rat25497
UniProt ID
HumanQ9UKX7
MouseQ9JIH2
RatO08587
Alternative Names
Nucleoporin 50; 50 KDa Nucleoporin; Nucleoporin 50kDa; Nucleoporin Nup50; NPAP60L; Nuclear Pore-Associated Protein 60 KDa-Like;
Function
Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:20016008).
Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:20016008).
Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity).
This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).
Biological Process
mRNA transportIEA:UniProtKB-KW
Nucleocytoplasmic transport1 PublicationIC:ComplexPortal
Protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus, nuclear pore complex
Nucleus membrane
Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity).
Dissociates from the NPC structure early during prophase of mitosis (PubMed:12802065).
Associates with the newly formed nuclear membrane during telophase (PubMed:12802065).
In the testis, the localization changes during germ cell differentiation from the nuclear surface in spermatocytes to the whole nucleus (interior) in spermatids and back to the nuclear surface in spermatozoa (By similarity).

Holzer, G., & Antonin, W. (2022). Nup50 plays more than one instrument. Cell Cycle, 21(17), 1785-1794.

Holzer, G., De Magistris, P., Gramminger, C., Sachdev, R., Magalska, A., Schooley, A., ... & Antonin, W. (2021). The nucleoporin Nup50 activates the Ran guanine nucleotide exchange factor RCC1 to promote NPC assembly at the end of mitosis. The EMBO Journal, 40(23), e108788.

Zheng, Y., Yang, Z., Jin, C., Chen, C., & Wu, N. (2021). hsa-miR-191-5p inhibits replication of human immunodeficiency virus type 1 by downregulating the expression of NUP50. Archives of Virology, 166, 755-766.

Megat, S., Mora, N., Sanogo, J., Catanese, A., Alami, N. O., Freischmidt, A., ... & Dupuis, L. (2021). Loss of nucleoporin Nup50 is a risk factor for amyotrophic lateral sclerosis. MedRxiv, 2021-08.

Holzer, G., de Magistris, P., Gramminger, C., Sachdev, R., Magalska, A., Schooley, A., ... & Antonin, W. (2021). The nucleoporin Nup50 activates the Ran guanyl-nucleotide exchange factor RCC1 to promote mitotic NPC assembly. BioRxiv, 2021-03.

De Magistris, P. (2019). Functional characterization of the nucleoporins Nup50 and Nup155 in postmitotic nuclear pore complex assembly (Doctoral dissertation, Universität Tübingen).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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