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Mouse Anti-NUP62 Recombinant Antibody (CBWJN-0433) (CBMAB-N0565-WJ)

This product is a Mouse antibody that recognizes NUP62. The antibody CBWJN-0433 can be used for immunoassay techniques such as: WB, IF, IP.
See all NUP62 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Chicken, Mouse, Rat
Clone
CBWJN-0433
Antibody Isotype
IgG2b
Application
WB, IF, IP

Basic Information

Specificity
Human, Chicken, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
0.25 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
nucleoporin 62kDa
Introduction
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human23636
Mouse18226
Rat65274
Chicken422183
UniProt ID
HumanP37198
MouseQ63850
RatP17955
ChickenF1NFA8
Alternative Names
Nucleoporin 62; Nuclear Pore Glycoprotein P62; 2 KDa Nucleoporin; Nucleoporin 62kDa; Nucleoporin Nup62; Nucleoporin 62kD; IBSN; SNDI; P62;
Function
Essential component of the nuclear pore complex (PubMed:1915414).
The N-terminal is probably involved in nucleocytoplasmic transport (PubMed:1915414).
The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed:1915414, PubMed:24107630).
Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed:24107630).
It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed:24107630).
Biological Process
Cell agingIEA:Ensembl
Cell deathManual Assertion Based On ExperimentIMP:UniProtKB
Cell surface receptor signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Centriole assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Centrosome cycleManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic centrosome separationManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic metaphase plate congressionManual Assertion Based On ExperimentIMP:UniProtKB
mRNA transportIEA:UniProtKB-KW
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of epidermal growth factor receptor signaling pathwayIEA:Ensembl
Negative regulation of MAP kinase activityIEA:Ensembl
Negative regulation of programmed cell deathManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of Ras protein signal transductionIEA:Ensembl
Nucleocytoplasmic transport1 PublicationIC:ComplexPortal
Positive regulation of centriole replicationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of epidermal growth factor receptor signaling pathway1 PublicationNAS:UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of mitotic cytokinetic processManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of mitotic nuclear divisionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein localization to centrosomeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central
Regulation of mitotic spindle organizationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of Ras protein signal transduction1 PublicationNAS:UniProtKB
Regulation of signal transduction1 PublicationNAS:UniProtKB
RNA export from nucleusManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus, nuclear pore complex
Cytoplasm, cytoskeleton, spindle pole
Nucleus envelope
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Central region of the nuclear pore, within the transporter (PubMed:1915414).
During mitotic cell division, it associates with the poles of the mitotic spindle (PubMed:24107630).
Involvement in disease
Infantile striatonigral degeneration (SNDI):
Neurological disorder characterized by symmetrical degeneration of the caudate nucleus, putamen, and occasionally the globus pallidus, with little involvement of the rest of the brain. The clinical features include developmental regression, choreoathetosis, dystonia, spasticity, dysphagia, failure to thrive, nystagmus, optic atrophy, and mental retardation.
PTM
O-glycosylated. Contains about 10 N-acetylglucosamine side chain sites predicted for the entire protein, among which only one in the C-terminal.
The inner channel of the NPC has a different redox environment from the cytoplasm and allows the formation of interchain disulfide bonds between some nucleoporins, the significant increase of these linkages upon oxidative stress reduces the permeability of the NPC.

Singh, U., Bindra, D., Samaiya, A., & Mishra, R. K. (2023). Overexpressed Nup88 stabilized through interaction with Nup62 promotes NF-κB dependent pathways in cancer. Frontiers in Oncology, 13, 1095046.

Chen, G., Xu, D., Liu, Q., Yue, Z., Dai, B., Pan, S., ... & Hu, H. (2023). Regulation of FLC nuclear import by coordinated action of the NUP62‐subcomplex and importin β SAD2. Journal of Integrative Plant Biology, 65(9), 2086-2106.

Yamazoe, K., & Inoue, Y. H. (2023). Cyclin B Export to the Cytoplasm via the Nup62 Subcomplex and Subsequent Rapid Nuclear Import Are Required for the Initiation of Drosophila Male Meiosis. Cells, 12(22), 2611.

Gleixner, A. M., Verdone, B. M., Otte, C. G., Anderson, E. N., Ramesh, N., Shapiro, O. R., ... & Donnelly, C. J. (2022). NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nature communications, 13(1), 3380.

Madheshiya, P. K., Shukla, E., Singh, J., Bawaria, S., Ansari, M. Y., & Chauhan, R. (2022). Insights into the role of Nup62 and Nup93 in assembling cytoplasmic ring and central transport channel of the nuclear pore complex. Molecular Biology of the Cell, 33(14), ar139.

Akbar, H., Cao, J., Wang, D., Yuan, X., Zhang, M., Muthusamy, S., ... & Liu, X. (2022). Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis. Journal of Molecular Cell Biology, 14(8), mjac056.

Nag, N., & Tripathi, T. (2022). Mislocalization of Nup62 contributes to TDP-43 proteinopathy in ALS/FTLD. ACS Chemical Neuroscience, 13(17), 2544-2546.

Chien, M. L., Lai, J. H., Lin, T. F., Yang, W. S., & Juang, Y. L. (2020). NUP62 is required for the maintenance of the spindle assembly checkpoint and chromosomal stability. The International Journal of Biochemistry & Cell Biology, 128, 105843.

Okazaki, R., Yamazoe, K., & Inoue, Y. H. (2020). Nuclear export of cyclin B mediated by the Nup62 complex is required for meiotic initiation in Drosophila males. Cells, 9(2), 270.

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For research use only. Not intended for any clinical use.

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