Mouse Anti-NUP85 Recombinant Antibody (CBWJN-1626) (CBMAB-N4022-WJ)

Name: Mouse Anti-NUP85 Recombinant Antibody (CBWJN-1626)
SKU: CBMAB-N4022-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBWJN-1626

Application

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nucleoporin 85kDa

Introduction

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Entrez Gene ID

Human	79902
Mouse	445007
Rat	287830

UniProt ID

Human	Q9BW27
Mouse	Q8R480
Rat	Q4QQS8

Alternative Names

Nucleoporin 85; Nucleoporin 85kDa; Nucleoporin Nup75; Nucleoporin Nup85; FROUNT; Nup75;

Function

Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance (PubMed:12718872).
As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus (PubMed:12718872).
The Nup107-160 complex seems to be required for spindle assembly during mitosis (PubMed:16807356).
NUP85 is required for membrane clustering of CCL2-activated CCR2 (PubMed:15995708).
Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade (PubMed:15995708).
Involved in nephrogenesis (PubMed:30179222).

Biological Process

Lamellipodium assemblyIEA:Ensembl
Macrophage chemotaxisIEA:Ensembl
mRNA export from nucleusManual Assertion Based On ExperimentIBA:GO_Central
Nephron developmentManual Assertion Based On ExperimentIMP:UniProtKB
Nucleocytoplasmic transport1 PublicationIC:ComplexPortal
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Protein import into nucleusManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus, nuclear pore complex
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, spindle
Cytoplasm
Nucleus membrane
During mitosis, localizes to the kinetochores and spindle poles (PubMed:12718872, PubMed:16807356).
Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells (PubMed:15995708).

Involvement in disease

Nephrotic syndrome 17 (NPHS17):
A form of nephrotic syndrome, a renal disease clinically characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form that progresses to end-stage renal failure. NPHS17 is an autosomal recessive, steroid-resistant progressive form with onset in the first decade of life.

Gambadauro, A., Mangano, G. D., Galletta, K., Granata, F., Riva, A., Massella, L., ... & Chimenz, R. (2023). NUP85 as a Neurodevelopmental Gene: From Podocyte to Neuron. Genes, 14(12), 2143.

Ravindran, E., Lesca, G., Januel, L., Goldgruber, L., & Kaindl, A. M. (2023). Case report: Compound heterozygous NUP85 variants cause autosomal recessive primary microcephaly. Frontiers in Neurology, 14, 1124886.

Zhang, M., Tian, S. Y., Ma, S. Y., Zhou, X., Zheng, X. H., Li, B., ... & Han, Y. (2023). Deficient chaperone‐mediated autophagy in macrophage aggravates inflammation of nonalcoholic steatohepatitis by targeting Nup85. Liver International, 43(5), 1021-1034.

Ravindran, E., Jühlen, R., Vieira-Vieira, C. H., Ha, T., Salzberg, Y., Fichtman, B., ... & Kaindl, A. M. (2021). Expanding the phenotype of NUP85 mutations beyond nephrotic syndrome to primary autosomal recessive microcephaly and Seckel syndrome spectrum disorders. Human molecular genetics, 30(22), 2068-2081.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
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For research use only. Not intended for any clinical use.

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