Mouse Anti-ODF2 (AA 706-805) Recombinant Antibody (1A1) (CBMAB-O0316-CQ)

This product is a mouse antibody that recognizes ODF2 (AA 706-805). The antibody 1A1 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all ODF2 antibodies

Published Data

Fig.1 hCenexin1 is the major Odf2-related isoform abundantly expressed at the late stages of the cell cycle.

Fig.2 Localization of ODF2 on human metaphase chromosomes.

Fig.3 Gene 111-450 encodes Odf84.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1A1

Antibody Isotype

IgG2a, Κ

Application

ELISA, IF, WB

Basic Information

Immunogen

Partial recombinant corresponding to aa706-805 from human ODF2 (NP_002531) with GST tag

Specificity

Human

Antibody Isotype

IgG2a, Κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 706-805

Target

Full Name

outer dense fiber of sperm tails 2

Introduction

The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers.

Entrez Gene ID

4957

UniProt ID

Q5BJF6

Alternative Names

Outer Dense Fiber Of Sperm Tails 2; Cancer/Testis Antigen 134; Cenexin; Testis Tissue Sperm-Binding Protein Li 51e; Outer Dense Fiber Of Sperm Tails Protein 2; Outer Dense Fiber Of Sperm Tails, 84-KD; Outer Dense Fibre Of Sperm Tails 2; Sperm Tail Structural Protein

Function

Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.

Biological Process

Centriole-centriole cohesionManual Assertion Based On ExperimentIGI:GO_Central
Cilium organizationManual Assertion Based On ExperimentIGI:ARUK-UCL
Protein localizationManual Assertion Based On ExperimentIMP:GO_Central
Regulation of cilium assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Spermatid developmentIEA:Ensembl

Cellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cell projection, cilium
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Cytoplasm, cytoskeleton, spindle pole
Cell projection, cilium, flagellum
Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.

PTM

Tyrosine phosphorylated. Phosphorylated by TSSK4 on Ser-95.

References

Xu, X., Jiang, X., Song, K., Zhang, J., Tian, Y., Chen, Y., ... & Ma, W. (2023). Stable expression and multi-site location of Odf2 in mouse oocytes, sperm and early embryos. Annals of Anatomy-Anatomischer Anzeiger, 250, 152126.

Zhu, Z. J., Wang, Y. Z., Wang, X. B., Yao, C. C., Zhao, L. Y., Zhang, Z. B., ... & Li, Z. (2022). Novel mutation in ODF2 causes multiple morphological abnormalities of the sperm flagella in an infertile male. Asian Journal of Andrology, 24(5), 463-472.

Kaya, A., Dogan, S., Vargovic, P., Kutchy, N. A., Ross, P., Topper, E., ... & Memili, E. (2022). Sperm proteins ODF2 and PAWP as markers of fertility in breeding bulls. Cell and Tissue Research, 387(1), 159-171.

Tapia Contreras, C., & Hoyer-Fender, S. (2019). CCDC42 localizes to manchette, HTCA and tail and interacts with ODF1 and ODF2 in the formation of the male germ cell cytoskeleton. Frontiers in cell and developmental biology, 7, 151.

Ito, C., Akutsu, H., Yao, R., Yoshida, K., Yamatoya, K., Mutoh, T., ... & Toshimori, K. (2019). Odf2 haploinsufficiency causes a new type of decapitated and decaudated spermatozoa, Odf2-DDS, in mice. Scientific reports, 9(1), 14249.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

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