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Rat Anti-OPN5 Recombinant Antibody (B5) (CBMAB-O0487-CQ)

This product is a rat antibody that recognizes OPN5. The antibody B5 can be used for immunoassay techniques such as: ICC, IHC, IP, Neut, WB.
See all OPN5 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
B5
Antibody Isotype
IgG2a
Application
ICC, IHC, IP, Neut, WB

Basic Information

Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, pH 7.2, 1% sucrose
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Opsin 5
Introduction
Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this family member may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
353344
UniProt ID
Q6VZZ7
Alternative Names
Opsin 5; Transmembrane Protein 13; Neuropsin; G-Protein Coupled Receptor 136; TMEM13; GPR136
Function
G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina (By similarity).
Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light) (PubMed:22043319).
Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake (By similarity).
Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock (By similarity).
Acts as a circadian photoreceptor in the outer ear, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle (By similarity).
Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (By similarity).
Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels (By similarity).
Biological Process
Cellular response to light stimulusISS:UniProtKB
Cellular response to UV-AManual Assertion Based On ExperimentIDA:UniProtKB
Entrainment of circadian clock by photoperiodISS:UniProtKB
G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Hyaloid vascular plexus regressionISS:UniProtKB
PhototransductionISS:UniProtKB
Phototransduction, UVManual Assertion Based On ExperimentIMP:UniProtKB
Protein-chromophore linkageIEA:UniProtKB-KW
Visual perceptionIEA:UniProtKB-KW
Cellular Location
Cell membrane
Topology
Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-74
Helical: 75-95
Extracellular: 96-108
Helical: 109-129
Cytoplasmic: 130-150
Helical: 151-171
Extracellular: 172-197
Helical: 198-218
Cytoplasmic: 219-252
Helical: 253-273
Extracellular: 274-288
Helical: 289-309
Cytoplasmic: 310-353
PTM
It is uncertain whether Cys-315 or Cys-316 is palmitoylated.

Jeong, H., Lee, D., Jiang, X., Negishi, K., Tsubota, K., & Kurihara, T. (2023). Opsin 5 mediates violet light-induced early growth response-1 expression in the mouse retina. Scientific reports, 13(1), 17861.

Karthikeyan, R., Davies, W. I., & Gunhaga, L. (2023). Non-image-forming functional roles of OPN3, OPN4 and OPN5 photopigments. Journal of Photochemistry and Photobiology, 15, 100177.

Sato, K., & Ohuchi, H. (2023). Molecular property, manipulation, and potential use of Opn5 and its homologs. Journal of Molecular Biology, 168319.

D'Souza, S. P., Swygart, D. I., Wienbar, S. R., Upton, B. A., Zhang, K. X., Mackin, R. D., ... & Lang, R. A. (2022). Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells. Journal of Comparative Neurology, 530(8), 1247-1262.

Lan, Y., Zeng, W., Dong, X., & Lu, H. (2021). Opsin 5 is a key regulator of ultraviolet radiation‐induced melanogenesis in human epidermal melanocytes. British Journal of Dermatology, 185(2), 391-404.

Jiang, X., Pardue, M. T., Mori, K., Ikeda, S. I., Torii, H., D’Souza, S., ... & Tsubota, K. (2021). Violet light suppresses lens-induced myopia via neuropsin (OPN5) in mice. Proceedings of the National Academy of Sciences, 118(22), e2018840118.

Yamashita, T. (2020). Unexpected molecular diversity of vertebrate nonvisual opsin Opn5. Biophysical Reviews, 12(2), 333-338.

Zhang, K. X., D’Souza, S., Upton, B. A., Kernodle, S., Vemaraju, S., Nayak, G., ... & Lang, R. A. (2020). Violet-light suppression of thermogenesis by opsin 5 hypothalamic neurons. Nature, 585(7825), 420-425.

Nguyen, M. T. T., Vemaraju, S., Nayak, G., Odaka, Y., Buhr, E. D., Alonzo, N., ... & Lang, R. A. (2019). An opsin 5–dopamine pathway mediates light-dependent vascular development in the eye. Nature cell biology, 21(4), 420-429.

Buhr, E. D., Vemaraju, S., Diaz, N., Lang, R. A., & Van Gelder, R. N. (2019). Neuropsin (OPN5) mediates local light-dependent induction of circadian clock genes and circadian photoentrainment in exposed murine skin. Current Biology, 29(20), 3478-3487.

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For research use only. Not intended for any clinical use.

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