Rabbit Anti-OS9 Recombinant Antibody (
26H3) (V2LY-0725-LY1534)

Name: Rabbit Anti-OS9 Recombinant Antibody (26H3)
SKU: V2LY-0725-LY1534
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-OS9 Recombinant Antibody (26H3) (V2LY-0725-LY1534) in WB

Western Blot
Positive WB detected in: HL-60 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue
All lanes: OS9 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 70, 68, 74, 66, 64, 70, 61 kDa
Observed band size: 72-95 kDa

Rabbit Anti-OS9 Recombinant Antibody (26H3) (V2LY-0725-LY1534) in IHC

IHC image of V2LY-0725-LY1534 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

26H3

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human OS9.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

OS9, Endoplasmic Reticulum Lectin

Entrez Gene ID

10956

UniProt ID

Q13438

Function

Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.

Biological Process

Endoplasmic reticulum unfolded protein responseIEA:InterPro
Negative regulation of retrograde protein transport, ER to cytosolManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Protein retention in ER lumenManual Assertion Based On ExperimentIDA:UniProtKB
Protein targetingIEA:Ensembl
Protein ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Response to endoplasmic reticulum stressManual Assertion Based On ExperimentIDA:UniProtKB
Retrograde protein transport, ER to cytosolManual Assertion Based On ExperimentIBA:GO_Central
Ubiquitin-dependent ERAD pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Endoplasmic reticulum lumen

PTM

Intramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated.

Frachon, N., Demaretz, S., Seaayfan, E., Chelbi, L., Bakhos-Douaihy, D., & Laghmani, K. (2024). AUP1 Regulates the Endoplasmic Reticulum-Associated Degradation and Polyubiquitination of NKCC2. Cells, 13(5), 389.

Erzurumlu, Y., Catakli, D., & Dogan, H. K. (2023). Circadian Oscillation Pattern of Endoplasmic Reticulum Quality Control (ERQC) Components in Human Embryonic Kidney HEK293 Cells. Journal of Circadian Rhythms, 21.

Li, P., Guo, Z., Feng, R., Wu, N., Zhong, X., Fang, Z., ... & Ge, J. (2022). Multi‐omics analysis reveals the regulation of SIRT6 on protein processing of endoplasmic reticulum to alleviate oxidative stress in endothelial cells. Clinical and Translational Medicine, 12(8).

Ninagawa, S., George, G., & Mori, K. (2021). Mechanisms of productive folding and endoplasmic reticulum-associated degradation of glycoproteins and non-glycoproteins. Biochimica et biophysica acta (BBA)-General subjects, 1865(3), 129812.

Wen, Y., Zong, S., Liu, T., Du, P., Li, H., & Xiao, H. (2021). Tauroursodeoxycholic acid attenuates cisplatin-induced ototoxicity by inhibiting the accumulation and aggregation of unfolded or misfolded proteins in the endoplasmic reticulum. Toxicology, 453, 152736.

Shaukat, I., Bakhos‐Douaihy, D., Zhu, Y., Seaayfan, E., Demaretz, S., Frachon, N., ... & Laghmani, K. (2021). New insights into the role of endoplasmic reticulum‐associated degradation in Bartter Syndrome Type 1. Human Mutation, 42(8), 947-968.

Lemmer, I. L., Willemsen, N., Hilal, N., & Bartelt, A. (2021). A guide to understanding endoplasmic reticulum stress in metabolic disorders. Molecular metabolism, 47, 101169.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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