Mouse Anti-OSBP Recombinant Antibody (5A3) (CBMAB-O0578-CQ)

Name: Mouse Anti-OSBP Recombinant Antibody (5A3)
SKU: CBMAB-O0578-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5A3

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2a, Κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

oxysterol binding protein

Introduction

Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase

Entrez Gene ID

5007

UniProt ID

P22059

Alternative Names

Oxysterol Binding Protein; OSBP1; Oxysterol-Binding Protein 1

Function

Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed:24209621).
Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed:15746430, PubMed:17428193).
Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed:15746430).
Regulates cholesterol efflux by decreasing ABCA1 stability (PubMed:18450749).

Biological Process

Bile acid biosynthetic processTAS:Reactome
Ceramide transportManual Assertion Based On ExperimentIMP:ComplexPortal
Intracellular cholesterol transportManual Assertion Based On ExperimentIMP:UniProtKB
Phospholipid transportManual Assertion Based On ExperimentIDA:ComplexPortal
Positive regulation of insulin secretion involved in cellular response to glucose stimulusIEA:Ensembl
Positive regulation of secretory granule organizationIEA:Ensembl
Positive regulation of tyrosine phosphorylation of STAT proteinIEA:Ensembl
Sphingomyelin biosynthetic processManual Assertion Based On ExperimentIMP:ComplexPortal
Sterol transportManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm, cytosol
Cytoplasm, perinuclear region
Golgi apparatus membrane ; Peripheral membrane protein
Endoplasmic reticulum membrane ; Peripheral membrane protein
Golgi apparatus, trans-Golgi network
Predominantly cytosolic.

Severance, Z. C., Nuñez, J. I., Le-McClain, A. T., Malinky, C. A., Bensen, R. C., Fogle, R. S., ... & Burgett, A. W. (2023). Structure–Activity Relationships of Ligand Binding to Oxysterol-Binding Protein (OSBP) and OSBP-Related Protein 4. Journal of medicinal chemistry, 66(6), 3866-3875.

Lin, Y., Ran, L., Du, X., Yang, H., & Wu, Y. (2023). Oxysterol-binding protein: New insights into lipid transport functions and human diseases. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 159365.

Bensen, R. C., Gunay, G., Finneran, M. C., Jhingan, I., Acar, H., & Burgett, A. W. (2021). Small molecule targeting of oxysterol-binding protein (OSBP)-related protein 4 and OSBP inhibits ovarian cancer cell proliferation in monolayer and spheroid cell models. ACS pharmacology & translational science, 4(2), 744-756.

Nakatsu, F., & Kawasaki, A. (2021). Functions of oxysterol-binding proteins at membrane contact sites and their control by phosphoinositide metabolism. Frontiers in Cell and Developmental Biology, 9, 664788.

Péresse, T., Kovacs, D., Subra, M., Bigay, J., Tsai, M. C., Polidori, J., ... & Mesmin, B. (2020). Molecular and cellular dissection of the oxysterol-binding protein cycle through a fluorescent inhibitor. Journal of Biological Chemistry, 295(13), 4277-4288.

Liu, H., & Huang, S. (2020). Role of oxysterol-binding protein-related proteins in malignant human tumours. World journal of clinical cases, 8(1), 1.

Ma, L., Zhang, X., Hu, Z., He, B., Ai, M., & Zeng, B. (2019). Heterologous expression and functional characterization of the ligand-binding domain of oxysterol-binding protein from Aspergillus oryzae. Brazilian Journal of Microbiology, 50, 415-424.

Roberts, B. L., Severance, Z. C., Bensen, R. C., Le-McClain, A. T., Malinky, C. A., Mettenbrink, E. M., ... & Burgett, A. W. (2019). Differing activities of oxysterol-binding protein (OSBP) targeting anti-viral compounds. Antiviral research, 170, 104548.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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