Mouse Anti-OTOA (AA 27-124) Recombinant Antibody (1F5) (CBMAB-O0689-CQ)

This product is a mouse antibody that recognizes OTOA (AA 27-124). The antibody 1F5 can be used for immunoassay techniques such as: ELISA, WB.
See all OTOA antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1F5

Antibody Isotype

IgG2a, Κ

Application

ELISA, WB

Basic Information

Immunogen

OTOA (NP_653273.3, 27aa-124aa) partial Recombinant protein with GST tag

Specificity

Human

Antibody Isotype

IgG2a, Κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 27-124

Target

Full Name

otoancorin

Introduction

The protein encoded by this gene is specifically expressed in the inner ear, and is located at the interface between the apical surface of the inner ear sensory epithelia and their overlying acellular gels. It is prposed that this protein is involved in the attachment of the inner ear acellular gels to the apical surface of the underlying nonsensory cells. Mutations in this gene are associated with autosomal recessive deafness type 22 (DFNB22). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

146183

UniProt ID

Q7RTW8

Alternative Names

Otoancorin; Cancer/Testis Antigen 108; Deafness, Autosomal Recessive 22; DFNB22; CT108

Function

May act as an adhesion molecule.

Biological Process

Cell-matrix adhesionManual Assertion Based On ExperimentIBA:GO_Central
Sensory perception of soundIEA:UniProtKB-KW
Transmission of nerve impulseIEA:Ensembl

Cellular Location

Apical cell membrane
Secreted, extracellular space, extracellular matrix
At the interface between the apical surface of the epithelia and the overlying acellular gel of the tectorial and otoconial membranes.

Involvement in disease

Deafness, autosomal recessive, 22 (DFNB22):
A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

References

Ortore, R. P., Leone, M. P., Palumbo, O., Petracca, A., Trecca, E. M., D’Ecclesia, A., ... & Castori, M. (2021). Compound Heterozygosity for OTOA Truncating Variant and Genomic Rearrangement Cause Autosomal Recessive Sensorineural Hearing Loss in an Italian Family. Audiology Research, 11(3), 443-451.

Zhang, L., Tian, W., & Zhou, B. (2020). Polymorphisms in Neuronal Growth Regulator 1 and Otoancorin Alternate the Susceptibility to Lung Cancer in Chinese Nonsmoking Females. DNA and Cell Biology, 39(9), 1657-1663.

Sugiyama, K., Moteki, H., Kitajiri, S. I., Kitano, T., Nishio, S. Y., Yamaguchi, T., ... & Usami, S. I. (2019). Mid-frequency hearing loss is characteristic clinical feature of OTOA-associated hearing loss. Genes, 10(9), 715.

Kim, B. J., Kim, D. K., Han, J. H., Oh, J., Kim, A. R., Lee, C., ... & Choi, B. Y. (2019). Clarification of glycosylphosphatidylinositol anchorage of OTOANCORIN and human OTOA variants associated with deafness. Human mutation, 40(5), 525-531.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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