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Mouse Anti-OTOP1 (AA 415-504) Recombinant Antibody (5E3) (CBMAB-O0692-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
5E3
Antibody Isotype
IgG2a, Κ
Application
ELISA, WB

Basic Information

Immunogen
OTOP1 (NP_819056.1, 415aa-504aa) partial Recombinant protein with GST tag
Specificity
Human
Antibody Isotype
IgG2a, Κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 415-504

Target

Full Name
otopetrin 1
Introduction
This gene encodes a transmembrane protein which belongs to the otopetrin domain protein family and is required for the formation of otoconia and otoliths, calcium carbonate biominerals within the inner ear of mammals that are required for the detection of linear acceleration and gravity. This gene modulates purinergic control of intracellular calcium in vestibular supporting cells. Naturally occurring mutations in the orthologous mouse gene are associated with nonsyndromic otoconia agenesis and a consequent balance defect. The orthologous mouse gene is also induced in white adipose tissue during obesity. The encoded protein is a component of a counterinflammatory pathway that attenuates obesity-induced adipose tissue inflammation and plays an adaptive role in maintaining metabolic homeostasis in obesity.
Entrez Gene ID
133060
UniProt ID
Q7RTM1
Alternative Names
Otopetrin 1; Otopetrin-1
Function
Proton-selective channel that specifically transports protons into cells (PubMed:29371428).
Proton channel activity is only weakly-sensitive to voltage (By similarity).
Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes (PubMed:29371428).
In the vestibular system of the inner ear, required for the formation and function of otoconia, which are calcium carbonate crystals that sense gravity and acceleration (By similarity).
Probably acts by maintaining the pH appropriate for formation of otoconia (By similarity).
Regulates purinergic control of intracellular calcium in vestibular supporting cells (By similarity).
May be involved in sour taste perception in sour taste cells by mediating entry of protons within the cytosol (By similarity).
Also involved in energy metabolism, by reducing adipose tissue inflammation and protecting from obesity-induced metabolic dysfunction (By similarity).
Biological Process
Biomineral tissue developmentIEA:UniProtKB-KW
Cellular response to insulin stimulusISS:UniProtKB
Detection of gravityIEA:Ensembl
Inner ear morphogenesisManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of interferon-gamma-mediated signaling pathwayISS:UniProtKB
Proton transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
More Infomation

Liang, Z., & Liman, E. R. (2024). Activation of the proton channel OTOP1 by ammonium chloride as a mechanism for taste transduction. Biophysical Journal, 123(3), 27a.

Liang, Z., Wilson, C. E., Teng, B., Kinnamon, S. C., & Liman, E. R. (2023). The proton channel OTOP1 is a sensor for the taste of ammonium chloride. Nature Communications, 14(1), 6194.

Tian, L., Zhang, H., Yang, S., Luo, A., Kamau, P. M., Hu, J., ... & Lai, R. (2023). Vertebrate OTOP1 is also an alkali-activated channel. Nature Communications, 14(1), 26.

Islam, M. M., Sasaki, O., Yano-Nashimoto, S., Okamatsu-Ogura, Y., & Yamaguchi, S. (2023). Cibacron blue 3G-A is a novel inhibitor of Otopetrin 1 (OTOP1), a proton channel. Biochemical and Biophysical Research Communications, 665, 64-70.

Li, B., Wang, Y., Castro, A., Ng, C., Wang, Z., Chaudhry, H., ... & Yu, Y. (2022). The roles of two extracellular loops in proton sensing and permeation in human Otop1 proton channel. Communications Biology, 5(1), 1110.

Khan, S. I., Della Santina, C. C., & Migliaccio, A. A. (2019). Angular vestibuloocular reflex responses in Otop1 mice. II. Otolith sensor input improves compensation after unilateral labyrinthectomy. Journal of neurophysiology, 121(6), 2300-2307.

Khan, S. I., Della Santina, C. C., & Migliaccio, A. A. (2019). Angular vestibuloocular reflex responses in Otop1 mice. I. Otolith sensor input is essential for gravity context-specific adaptation. Journal of neurophysiology, 121(6), 2291-2299.

Saotome, K., Teng, B., Tsui, C. C., Lee, W. H., Tu, Y. H., Kaplan, J. P., ... & Ward, A. B. (2019). Structures of the otopetrin proton channels Otop1 and Otop3. Nature structural & molecular biology, 26(6), 518-525.

Teng, B., Wilson, C. E., Tu, Y. H., Joshi, N. R., Kinnamon, S. C., & Liman, E. R. (2019). Cellular and neural responses to sour stimuli require the proton channel Otop1. Current Biology, 29(21), 3647-3656.

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For research use only. Not intended for any clinical use.

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