Mouse Anti-P2RY1 Recombinant Antibody (4C2) (CBMAB-P0593-YC)

Provided herein is a Mouse monoclonal antibody against Human Purinergic Receptor P2Y1. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all P2RY1 antibodies

Published Data

Fig.1 Western blot analysis for P2RY1.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

4C2

Antibody Isotype

IgG2a, κ

Application

ELISA, IF, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Purinergic Receptor P2Y1

Introduction

P2RY1 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation.

Entrez Gene ID

5028

UniProt ID

P47900

Alternative Names

Purinergic Receptor P2Y1; Suppressing Androgen Receptor In Renal Cell Carcinoma; Purinergic Receptor P2Y, G-Protein Coupled, 1; ATP Receptor; P2Y1; P2 Purinoceptor Subtype Y1;

Function

Receptor for extracellular adenine nucleotides such as ADP (PubMed:9442040, PubMed:9038354, PubMed:25822790).
In platelets, binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and ultimately platelet aggregation (PubMed:9442040).

Biological Process

Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathwayIEA:Ensembl
AgingIEA:Ensembl
Blood vessel diameter maintenanceIEA:Ensembl
Cell surface receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Cellular response to ATPManual Assertion Based On ExperimentTAS:ARUK-UCL
Cellular response to purine-containing compoundManual Assertion Based On ExperimentIMP:UniProtKB
Eating behaviorIEA:Ensembl
G protein-coupled adenosine receptor signaling pathwayIEA:Ensembl
G protein-coupled receptor signaling pathwayISS:BHF-UCL
Glial cell migrationIEA:Ensembl
Negative regulation of bindingIEA:Ensembl
Negative regulation of norepinephrine secretionIEA:Ensembl
Phospholipase C-activating G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Platelet activationIEA:InterPro
Positive regulation of cytosolic calcium ion concentrationISS:BHF-UCL
Positive regulation of ERK1 and ERK2 cascadeISS:BHF-UCL
Positive regulation of hormone secretionIEA:Ensembl
Positive regulation of inositol trisphosphate biosynthetic processIEA:Ensembl
Positive regulation of ion transportIEA:Ensembl
Positive regulation of penile erectionIEA:Ensembl
Positive regulation of protein phosphorylationISS:BHF-UCL
Positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Protein localization to plasma membraneISS:BHF-UCL
Regulation of cell shapeManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of presynaptic cytosolic calcium ion concentrationIEA:Ensembl
Regulation of signaling receptor activityIEA:Ensembl
Regulation of synaptic vesicle exocytosisIEA:Ensembl
Relaxation of muscleIEA:InterPro
Response to growth factorIEA:Ensembl
Response to mechanical stimulusIEA:Ensembl
Sensory perception of painIEA:Ensembl
Signal transduction involved in regulation of gene expressionISS:BHF-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 1-51
Helical: 52-74
Cytoplasmic: 75-87
Helical: 88-109
Extracellular: 110-125
Helical: 126-147
Cytoplasmic: 148-166
Helical: 167-188
Extracellular: 189-214
Helical: 215-237
Cytoplasmic: 238-260
Helical: 261-284
Extracellular: 285-303
Helical: 304-325
Cytoplasmic: 326-373

References

Dance, A., Fernandes, J., Toussaint, B., Vaillant, E., Boutry, R., Baron, M., ... & Bonnefond, A. (2024). Exploring the role of purinergic receptor P2RY1 in type 2 diabetes risk and pathophysiology: Insights from human functional genomics. Molecular metabolism, 79, 101867.

Christopher, R., Sundaravadivel, P., Chandra, S. R., & Ramakrishnan, S. (2024). Platelet ADP receptor (P2RY1) polymorphism and the risk of inadequate platelet response to aspirin: Study in patients with ischemic stroke.

Hua, Y., Liu, Y., Li, L., & Liu, G. (2023). Activation of hypermethylated P2RY1 mitigates gastric cancer by promoting apoptosis and inhibiting proliferation. Open Life Sciences, 18(1), 20220078.

Mouradian, G., Cook-Snyder, D., Dir, B., Segar, J., Hodges, M., & Grobe, J. (2023). The NEB-P2ry1 lung-brainstem axis and the control of breathing in a mouse model of bronchopulmonary dysplasia. Physiology, 38(S1), 5734221.

Moayedi, Y., Pitman, M. J., & de Nooij, J. C. (2020). Airway Protection—A Role for Vagal P2RY1 Receptors. Cell, 181(3), 509-511.

Liu, J., Hu, Y. S., & Tang, Y. (2020). Commentary: Vagal P2RY1 receptors: a novel target for airway disease. Frontiers in Pharmacology, 11, 596003.

Tan, Y., Zhang, T., Zhou, L., Liu, S., & Liang, C. (2019). MiR-34b-3p represses the multidrug-chemoresistance of bladder cancer cells by regulating the CCND2 and P2RY1 genes. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 25, 1323.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-P2RY1 Recombinant Antibody (4C2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: