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Rat Anti-PACSIN1 Recombinant Antibody (10A6B55) (CBMAB-P0631-YC)


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Summary

Host Animal
Rat
Specificity
Mouse
Clone
10A6B55
Antibody Isotype
IgG2b, κ
Application
WB, IP

Basic Information

Immunogen
Partial mouse PACSIN1 recombinant protein (26-275 a.a.) expressed in E. coli
Specificity
Mouse
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.2, containing 0.09% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Protein Kinase C And Casein Kinase Substrate In Neurons 1
Introduction
PACSIN1 has biased expression in brain (RPKM 42.1) and lymph node (RPKM 1.5).
Entrez Gene ID
23969
UniProt ID
Q61644
Alternative Names
Protein Kinase C And Casein Kinase Substrate In Neurons 1; Syndapin I; Syndapin-1; Protein Kinase C And Casein Kinase Substrate In Neurons Protein 1; KIAA1379; SDPI;
Function
Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity).
Binds to membranes via its F-BAR domain and mediates membrane tubulation.
Biological Process
Actin filament organizationIEA:InterPro
Cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of endocytosisIEA:Ensembl
Neuron projection morphogenesisISS:UniProtKB
Plasma membrane tubulationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of dendrite developmentISS:BHF-UCL
Protein localization to membraneISS:UniProtKB
Protein localization to plasma membraneISS:BHF-UCL
Regulation of endocytosisManual Assertion Based On ExperimentIBA:GO_Central
Synaptic vesicle endocytosisISS:UniProtKB
Cellular Location
Cytoplasm
Cell projection
Cell junction, synapse, synaptosome
Cell projection, ruffle membrane
Membrane
Cytoplasmic vesicle membrane
Cell junction, synapse
Cytoplasm, cytosol
Cell membrane
Colocalizes with MAPT in axons. In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites (By similarity).
Associates with membranes via its F-BAR domain.
PTM
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
More Infomation

Martellucci, S., Flütsch, A., Carter, M., Norimoto, M., Pizzo, D., Mantuano, E., ... & Campana, W. M. (2024). Axon‐derived PACSIN1 binds to the S chwann cell survival receptor, LRP1, and transactivates TrkC to promote gliatrophic activities. Glia.

Shi, Y., Zhao, K., Yang, G., Yu, J., Li, Y., Kessels, M. M., ... & Yang, S. N. (2022). Inositol hexakisphosphate primes syndapin I/PACSIN 1 activation in endocytosis. Cellular and Molecular Life Sciences, 79(6), 286.

Oe, Y., Kakuda, K., Yoshimura, S. I., Hara, N., Hasegawa, J., Terawaki, S., ... & Nakamura, S. (2022). PACSIN1 is indispensable for amphisome-lysosome fusion during basal autophagy and subsets of selective autophagy. PLoS Genetics, 18(6), e1010264.

Regan, P., Mitchell, S. J., Kim, S. C., Lee, Y., Yi, J. H., Barbati, S. A., ... & Cho, K. (2021). Regulation of synapse weakening through interactions of the microtubule associated protein tau with PACSIN1. Journal of Neuroscience, 41(34), 7162-7170.

Zimu, Z., Jia, Z., Xian, F., Rui, M., Yuting, R., Yuan, W., ... & Enfang, S. (2021). Decreased expression of PACSIN1 in brain glioma samples predicts poor prognosis. Frontiers in Molecular Biosciences, 8, 696072.

Liu, Z., Li, L., Li, X., Hua, M., Sun, H., & Zhang, S. (2021). Prediction and prognostic significance of ALOX12B and PACSIN1 expression in gastric cancer by genome-wide RNA expression and methylation analysis. Journal of Gastrointestinal Oncology, 12(5), 2082.

Boll, E., Cantrelle, F. X., Landrieu, I., Hirel, M., Sinnaeve, D., & Levy, G. (2020). 1 H, 13 C, and 15 N chemical shift assignment of human PACSIN1/syndapin I SH3 domain in solution. Biomolecular NMR Assignments, 14, 175-178.

Mahmood, M. I., Noguchi, H., & Okazaki, K. I. (2019). Curvature induction and sensing of the F-BAR protein Pacsin1 on lipid membranes via molecular dynamics simulations. Scientific Reports, 9(1), 14557.

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For research use only. Not intended for any clinical use.

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